Advancing 'omics analysis with a Sciex ZenoToF 7600 mass spectrometer

使用 Sciex ZenoToF 7600 质谱仪推进组学分析

• 批准号: BB/W019892/1
• 负责人: Katherine Hollywood
• 金额: $ 90.74万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW019892%2F1
• 关键词: Advancing; omics; analysis; Sciex; ZenoToF

Understanding the complexity of biology, and engineering biology for our benefit, requires the analysis of a wide range of biomolecules with high accuracy and precision. Mass spectrometry is one of the few techniques capable of providing an insight into this biological complexity, and the data we can generate with mass spectrometry can be used to increase our understanding of how biological systems work. However, the range of molecules important in biology is bewildering. They can be very small, for example the metabolites involved in the conversion of glucose to energy in the cell, to very large, for example the enzymes that catalyse the chemical reactions that make all the other biomolecules. They can also be very stable, such as the collagen that provides the support for the cells in our body or some of the components that make up fats, or very fragile, such as many of the structures added to proteins to control their activity or localization in the cell.This project is to provide a new generation mass spectrometer that will allow us to perform the complex analysis required to confidently identify the structures of, and measure the amount of many of the molecules that are present in complex biological samples. This is based on improvements in the way that molecules are fragmented in the mass spectrometer to provide information on their structure, enable discrimination between molecules that are only subtly different in structure and provide accurate quantification. The equipment is the first commercial time of flight mass spectrometer that has electron activated dissociation (EAD), a way of fragmenting molecules that is very fast and so gives much more information on structure than other methods, and is able to vary the energy of this fragmentation. Low energy EAD is especially valuable for molecules which have labile chemical groups, as the speed of fragmentation ensures it is not just these that are seen, and high energy EAD allows fragmentation of very stable molecules. It also has improvements that allows small to large fragments to be measured sensitively at the same time, which is critical for large biomolecules and for fast and accurate quantification of specific molecules in complex mixtures.This instrument will provide benefit to many research programs at the University of Manchester (UoM) in important strategic areas for the country, and in collaboration with a number of major pharmaceutical and biotechnology companies. This includes substantial research in biotechnology, for example in biofuels production and bio-feedstock utilization, and in the development of clean biological catalysts for sustainable chemical production. It also benefits bioscience for health, for example in understanding how the utilization of fats and lipids changes in Parkinson's disease and how inflammation adversely affects biomolecules, and bioscience for sustainable agriculture and food, for example in identifying and understanding the generation of food-based allergens during food processing. The new instrument will generate world-class and underpinning bioscience, for example in the development of analytical technologies and methodologies to understand better the way enzymes work and how biological systems function, and engineering biological processes to use microorganisms to make new chemicals.

了解生物学的复杂性，并为我们的利益工程生物学，需要高精度和高精度的分析范围广泛的生物分子。质谱是少数几种能够深入了解这种生物复杂性的技术之一，我们可以用质谱产生的数据来增加我们对生物系统如何工作的理解。然而，在生物学中重要的分子的范围是令人困惑的。它们可以非常小，例如参与细胞中葡萄糖转化为能量的代谢物，也可以非常大，例如催化制造所有其他生物分子的化学反应的酶。它们也可以是非常稳定的，例如为我们体内的细胞提供支持的胶原蛋白或构成脂肪的一些成分，或者非常脆弱，例如添加到蛋白质中以控制其活性或在细胞中定位的许多结构。该项目是提供新一代质谱仪，使我们能够进行复杂的分析，以确信地识别蛋白质的结构，并测量复杂生物样品中存在的许多分子的量。这是基于分子在质谱仪中被片段化的方式的改进，以提供关于其结构的信息，使得能够区分在结构上仅细微不同的分子并提供准确的定量。该设备是第一个具有电子活化解离（EAD）的商业飞行时间质谱仪，这是一种非常快速的分子碎片化方法，因此比其他方法提供更多的结构信息，并且能够改变这种碎片化的能量。低能量EAD对于具有不稳定化学基团的分子特别有价值，因为碎片化的速度确保它不仅仅是那些被看到的分子，而高能量EAD允许非常稳定的分子碎片化。它还具有改进，允许同时灵敏地测量小到大的片段，这对于大生物分子以及复杂混合物中特定分子的快速准确定量至关重要。该仪器将为曼彻斯特大学（UoM）在国家重要战略领域的许多研究项目提供益处，并与一些主要的制药和生物技术公司合作。这包括在生物技术方面进行大量研究，例如在生物燃料生产和生物原料利用方面，以及在为可持续化学品生产开发清洁生物催化剂方面。它还有利于健康生物科学，例如了解帕金森病中脂肪和脂质的利用如何变化以及炎症如何对生物分子产生不利影响，以及可持续农业和食品生物科学，例如识别和了解食品加工过程中食品过敏原的产生。新仪器将产生世界级的生物科学基础，例如开发分析技术和方法，以更好地了解酶的工作方式和生物系统的功能，并设计生物过程，利用微生物制造新的化学品。