GENETIC ANALYSIS OF NEMATODE DEVELOPMENT

这项工作的广泛目标是了解遗传和分子 细胞分裂和分化的时间控制机制 线虫C.优雅的模特 基因和分子分析将 进行，以确定如何调节途径的异时 基因控制着不同的时间协调表达， C.发育事件线虫幼虫 重点将放在 了解某些基因是如何控制不同生物体中事件发生的时间的， 细胞类型，以及其他基因如何发挥作用，以指定执行 特殊事件。 基因筛选将用于识别新的 这条路上的基因 将对关键基因进行分子分析， 决定其产品的结构和表达，以及它们如何 与其他异时基因的产物相互作用。 镶嵌分析 将用于评估这些基因在细胞间信号传导中的作用 协调不同细胞中发育程序的过程 血统 重点将放在理解分子 lin-14是一个在控制中起核心作用的基因， 不同的幼虫细胞谱系，是由新的调节 由lin-4编码的分子。 此外，一项主要工作将集中在 了解lin-29编码的转录因子是如何 发育调节，以及它如何控制转录， 成虫特异性基因和幼虫特异性基因。 一般来说，C. 线虫异时基因提供了一个研究遗传和 控制细胞分裂和分化的分子机制， 所有多细胞发育的核心过程。 更具体地说， 异时基因影响发育时间的事实 提供了一个独特的系统，在其中详细审查监管 时间控制的潜在机制。

The broad goal of this work is to understand the genetic and molecular mechanisms of temporal control of cell division and differentiation using the nematode C. elegans as a model. Genetic and molecular analysis will be performed to determine how the regulatory pathway of heterochronic genes controls the temporally coordinated expression of diverse developmental events in C. elegans larvae. Emphasis will be placed on understanding how certain genes control the timing of events in diverse cell types, and how other genes act to specify the execution of particular events. Genetic screens will be employed to identify new genes in this pathway. Key genes will be analysed molecularly to determine the structure and expression of their products, and how they interact with the products of other heterochronic genes. Mosaic analysis will be used to assess the role of these genes in cell-cell signalling processes that coordinate developmental programs in separate cell lineages. Emphasis will be placed on understanding the molecular mechanism of how lin-14, a gene that plays a central role in the control of diverse larval cell lineages, is regulated by the novel regulatory molecule encoded by lin-4. Also a major effort will be focused on understanding how the transcription factor encoded by lin-29 is developmentally regulated, and how it controls the transcription of adult-specific and larval-specific genes. Generally speaking, the C. elegans heterochronic genes offer an opportunity to study the genetic and molecular mechanisms controlling cell division and differentiation, processes central to all multicellular development. More specifically, the fact that the heterochronic genes affect developmental timing provides a unique system in which to examine in detail the regulatory mechanisms underlying temporal control.