Developmental Regulation of MicroRNA Expression

MicroRNA 表达的发育调控

• 批准号: 7933637
• 负责人: Victor Ambros
• 金额: $ 38.57万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-24 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7933637
• 关键词: Animals; Binding; Biogenesis; Biological Models; Caenorhabditis elegans; DNA Sequence; Development; Elements; Family; Gene Expression; Genes; Goals; Health; Homologous Gene; Human; Mammals; MicroRNAs; Molecular; Molecular Genetics; Nematoda; Pathway interactions; Pattern; Play; Process; Proteins; RNA; Regulation; Regulatory Pathway; Role; Signal Transduction; System; Time; Transcript; Transcriptional Regulation; Work; base; design; genetic analysis; genome wide association study; genome-wide; human disease; public health relevance; transcription factor

The broad goal of this work is to use the C. elegans model system to understand the genetic and molecular mechanisms underlying the developmental regulation of microRNA expression. There are more than 150 distinct microRNA genes in C. elegans, including more than 20 families of microRNAs with homologs in humans. The functions of these are largely unknown, although it is clear that microRNAs play important roles in a wide spectrum of developmeental and phsyiologocal processes in all animals. Little is known about how microRNA gene expression is regulated transcriptionaly or post-transcriptionally by developmental and physiologiical signals. Molecular and genetic approaches will be employed to characterize mechanisms of microRNA transcriptional regulation in C.elegans, and to characterize the proteins and RNA elements responsible for post-transcriptional developmental regulation of microRNA biogenesis.

这项工作的主要目标是使用C。elegans模型系统来理解遗传 以及microRNA表达的发育调控的分子机制。 在C.包括20多个 与人类同源的microRNA家族。它们的功能在很大程度上是未知的， 尽管很明显microRNA在广泛的发育过程中起着重要的作用， 和生理过程。关于microRNA基因是如何 表达在转录或转录后受发育和 生理信号。将采用分子和遗传学方法来表征 线虫microRNA转录调控机制的研究， 负责转录后发育调控的蛋白质和RNA元件 microRNA生物发生