BACTERIAL CELL MEMBRANE: GROWTH AND CELL DIVISION

细菌细胞膜：生长和细胞分裂

• 批准号: 3284661
• 负责人: MOSELIO SCHAECHTER
• 金额: $ 16.76万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3284661
• 关键词: Escherichia coli; Salmonella typhimurium; bacterial DNA; bacterial genetics; bacteriophage lambda; cell cycle; cell growth regulation; cell membrane; density gradient ultracentrifugation; electron microscopy; gel electrophoresis; membrane structure; microorganism growth; mutant; plasmids; radiotracer

We have recently succeeded in isolating the replicative origin of the Escherichia coli chromosome. The complex is composed of the DNA at and near the origin, and various proteins. We call this complex OC, for "origin complex". We will attempt further characterization of the OC particles. specifically, we will purify them with regard to protein content, and attempt to characterize the intrinsic proteins of these particles. Preliminary results have shown that partially purified DNA from OC binds to outer membrane, while other DNA does not. We will use a filter-binding assay to determine further the specificity and other properties of this interaction.

我们最近成功地分离出了 大肠杆菌染色体。 该复合物由位于和靠近 起源和各种蛋白质。 我们称这种复合体为OC，即“起源复合体”。 我们将尝试进一步表征OC颗粒。 我们特别 将纯化它们的蛋白质含量，并试图表征 这些颗粒的内在蛋白质。 初步结果显示， OC中部分纯化的DNA与外膜结合，而其他DNA则与外膜结合。 没有 我们将使用滤膜结合试验进一步确定特异性 以及这种相互作用的其他特性。