METALLO MONOOXYGENASE CATALYSIS

金属单加氧酶催化

• 批准号: 3284179
• 负责人: SHELDON W MAY
• 金额: $ 10.92万
• 依托单位: GEORGIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-08-01 至 1987-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3284179
• 关键词: Pseudomonas; chemical binding; enzyme structure; enzyme substrate; enzyme substrate analog; epoxides; iron; nuclear magnetic resonance spectroscopy; oxygenases; respiratory oxygenation; rubredoxins; stereochemistry

This proposal focuses on the oxygenation reactions carried out by the non-heme-iron-containing monooxygenase, the epoxidation/hydroxylation system of P. oleovorans. The significance of oxygenases--enzymes which catalyze the direct insertion of molecular oxygen into organic substrates--to biochemistry, medicine, and considerations of public health has by now been very well established. While P-450 and flavin-containing oxygenases have been intensively studied on a molecular level, the state of our understanding of non-heme iron monooxygenase catalysis is, by comparison, poor indeed. Thus, this project represents one of the very few efforts to define the molecular details of non-heme iron monooxygenase catalysis. Our work will involve mechanistic studies, the design and evaluation of novel substrate analogs, metal replacement and other active-site-directed chemical techniques and, finally, initial attempts to apply physical techniques to non-heme iron monooxygenases.

这项建议侧重于由 非血红素铁单加氧酶，环氧化/羟化 奥氏松毛虫的系统。加氧酶的意义--酶 催化分子氧直接插入有机化合物 底物--生物化学、医学和公共卫生方面的考虑 到目前为止已经非常成熟了。而P-450和含有黄素的 加氧酶已经在分子水平上得到了深入的研究，加氧酶的状态 我们对非血红素铁单加氧酶催化的理解是 相比之下，确实很可怜。因此，这个项目是为数不多的 确定非血红素铁单加氧酶分子细节的努力 催化作用。我们的工作将涉及机械研究、设计和 新型底物类似物、金属替代物和其他的评价 活性部位导向的化学技术，最后，初步尝试 将物理技术应用于非血红素铁单加氧酶。