INITIATION OF LAGGING-STRAND DNA SYNTHESIS

滞后链 DNA 合成的起始

基本信息

  • 批准号:
    3285791
  • 负责人:
  • 金额:
    $ 16.77万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1984
  • 资助国家:
    美国
  • 起止时间:
    1984-07-01 至 1987-12-31
  • 项目状态:
    已结题

项目摘要

The proposed work will continue our laboratory's effort to detail the events that occur during the initiation of lagging-strand DNA synthesis and the subsequent maintenance of the priming apparatus on the DNA. Two regions of pBR322 DNA have been shown to act as effector DNAs for the singlestrand DNA-dependent ATPase activity of replication factor Y (Zipursky and Marians, PNAS, 77, 6521-6525 (1980)), a pre-priming E. coli replication protein which directs the assembly of the primosome. We have demonstrated that the factor Y effector sites of pBR322 DNA can act as origins of complementary strand synthesis when cloned into single-strand phage vectors (Zipursky and Marians, PNAS, 78, 6111-6115(1981). The proposed work can be divided into two major sections. We will first study the interaction of factor Y with its effector sites on a molecular level. Through the use of DNA footprinting, methylation enhancement and in vitro mutant construction we will determine precisely those bases within a factor Y effector site which are required for origin function. This work will define the mechanism of interaction between factor Y and its effector DNAs and define the requirements for a region of DNA to function as a lagging-strand origin of replication. The second section of the proposed work is biochemical in nature. Reconstitution of ss(c) greater than RF DNA synthesis with purified proteins using the recombinant Y-site-fl phage DNAs as templates will allow us to determine which proteins are involved, the direction of primosome movement, and the role of the ATP hydrolyzing proteins (factor Y and the dnaB protein) in unwinding duplex DNA during replication.
拟议的工作将继续我们实验室的努力,详细说明

项目成果

期刊论文数量(0)
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专利数量(0)

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KENNETH J MARIANS其他文献

KENNETH J MARIANS的其他文献

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{{ truncateString('KENNETH J MARIANS', 18)}}的其他基金

Mechanisms of DNA Replication, Chromosome Compaction, and Chromosome Unlinking
DNA 复制、染色体压缩和染色体解联机制
  • 批准号:
    10618506
  • 财政年份:
    2018
  • 资助金额:
    $ 16.77万
  • 项目类别:
Mechanisms of DNA Replication, Chromosome Compaction, and Chromosome Unlinking
DNA 复制、染色体压缩和染色体解联机制
  • 批准号:
    9900025
  • 财政年份:
    2018
  • 资助金额:
    $ 16.77万
  • 项目类别:
Mechanisms of DNA Replication, Chromosome Compaction, and Chromosome Unlinking
DNA 复制、染色体压缩和染色体解联机制
  • 批准号:
    10373984
  • 财政年份:
    2018
  • 资助金额:
    $ 16.77万
  • 项目类别:
Topoisomerases and Chromosome Segregation
拓扑异构酶和染色体分离
  • 批准号:
    7988465
  • 财政年份:
    2009
  • 资助金额:
    $ 16.77万
  • 项目类别:
Integrated PhD Training Program in Cancer Biology
癌症生物学综合博士培训计划
  • 批准号:
    7293596
  • 财政年份:
    2006
  • 资助金额:
    $ 16.77万
  • 项目类别:
Integrated PhD Training Program in Cancer Biology
癌症生物学综合博士培训计划
  • 批准号:
    7492914
  • 财政年份:
    2006
  • 资助金额:
    $ 16.77万
  • 项目类别:
Integrated PhD Training Program in Cancer Biology
癌症生物学综合博士培训计划
  • 批准号:
    7220759
  • 财政年份:
    2006
  • 资助金额:
    $ 16.77万
  • 项目类别:
Integrated PhD Training Program in Cancer Biology
癌症生物学综合博士培训计划
  • 批准号:
    7669223
  • 财政年份:
    2006
  • 资助金额:
    $ 16.77万
  • 项目类别:
Conference on DNA Replication and Recombination
DNA复制与重组会议
  • 批准号:
    6434547
  • 财政年份:
    2002
  • 资助金额:
    $ 16.77万
  • 项目类别:
MOLECULAR BIOLOGY
分子生物学
  • 批准号:
    6563635
  • 财政年份:
    2002
  • 资助金额:
    $ 16.77万
  • 项目类别:

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钠钾转运三磷酸腺苷酶的分子表征
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    7301506
  • 财政年份:
    1973
  • 资助金额:
    $ 16.77万
  • 项目类别:
    Continuing Grant
MOLECULAR CHARACTERIZATION OF THE SODIUM-POTASSIUM TRANSPORT ADENOSINETRIPHOSPHATASE
钠钾转运腺苷三磷酸酶的分子表征
  • 批准号:
    7243716
  • 财政年份:
    1972
  • 资助金额:
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  • 财政年份:
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  • 资助金额:
    $ 16.77万
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钠钾转运三磷酸腺苷酶的分子表征
  • 批准号:
    6928993
  • 财政年份:
    1969
  • 资助金额:
    $ 16.77万
  • 项目类别:
Adenosinetriphosphatase Genesis in Bone Marrow Cells
骨髓细胞中腺苷三磷酸酶的发生
  • 批准号:
    64B2295
  • 财政年份:
    1964
  • 资助金额:
    $ 16.77万
  • 项目类别:
Adenosinetriphosphatase and sugar Transport Mechanism
三磷酸腺苷酶和糖转运机制
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  • 财政年份:
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    $ 16.77万
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骨髓细胞及其与三磷酸腺苷酶活性的关系
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    6216803
  • 财政年份:
    1962
  • 资助金额:
    $ 16.77万
  • 项目类别:
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