SURFACE ENHANCED RAMAN STUDIES OF BIOMOLECULES

生物分子的表面增强拉曼研究

• 批准号: 3287229
• 负责人: THERESE M COTTON
• 金额: $ 2.95万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3287229
• 关键词: Raman spectrometry; aminoacid; biophysics; chemical structure; copper; cytochrome c; electrochemistry; electrodes; enzyme electrodes; gold; lipids; macromolecule; membrane activity; molecular biology; molecular energy level; molecular polarity; molecular size; molecular stacking; molecular weight; photosynthesis; phycobilin; proteins; silver; tin

The goal of this research is to develop surface enhanced Raman scattering (SERS) and surface enhanced resonance Raman scattering (SERRS) techniques for the study of biomolecules. During this funding period there are four specific aims. The first is to continue the development of new substrates for SERS/SERRS. This will include the use of Au and Cu as well as Ag in the form of electrodes, thin films, and coated spheres of known size distribution. The application of SERS to the study of chemically modified surfaces (especially SnO2 and carbon) will also be continued. Modification will be accomplished by spontaneous adsorption, deposition of Langmuir Blodgett monolayers, and covalent bonding. The second aim is to pursue SERS investigations of amino acids, proteins, and lipids on the above named metals, as well as the modified electrode surfaces. The objective is to identify specific interactions between these molecules and the electrode surfaces. The application of SERRS to mechanistic studies of electron transfer proteins constitutes the third aim. Methods for the evaluation of activity and structure in flavoenzymes and cytochrome c will be sought. New procedures for adsorbing these proteins to electrode surfaces will be attempted in an effort to preserve their native structure. A number of proteins containing chromosphores will also be examined by SERRS. These will include photosynthetic preparations, phytochrome, and phycocyanin. The goal of these studies is to utilize the surface sensitivity of SERRS to determine structural information about the proteins. The fourth aim is to pursue purely analytical applications of SERS/SERRS. This will include detection of proteins separated by chromatography. Methods for detecting the interaction of small chromophoric molecules with capture proteins will also be investigated. In all of the above studies, an effort will be made to increase the sensitivity of the surface Raman technique through the use of new experimental approaches (i.e. fiber optics, attenuated total reflectance and micro Raman sampling procedures). Advances in detector technology will also be important with respect to this goal.

本研究的目标是发展表面增强拉曼光谱。 散射(SERS)和表面增强共振拉曼 生物分子研究中的散射(SERRS)技术。 在这一筹资期间，有四个具体目标。第一 是继续开发新的衬底，以满足 SERS/SERRS。这将包括金和铜的使用以及 电极、薄膜和涂覆的球体形式的银 已知的大小分布。表面增强拉曼光谱在化学发光研究中的应用 经过化学修饰的表面(特别是SnO2和碳)将 也将继续。修改将通过以下方式完成 朗缪尔·布洛杰特的自发吸附和沉积 单分子膜和共价键。第二个目标是追求 大豆中氨基酸、蛋白质和脂类的SERS研究 上述金属，以及修饰电极表面。 目标是确定这些组件之间的具体交互作用 分子和电极表面。 SerRS在电子力学研究中的应用 转移蛋白构成了第三个目标。的方法。 黄素酶活性和结构的评价 将寻找细胞色素c。吸附这些物质的新方法 蛋白质到电极表面的尝试将努力 保护它们原有的结构。含有多种蛋白质的 染色体也将由SERRS进行检查。这些遗嘱 包括光合作用制剂、光敏色素和 藻蓝蛋白。这些研究的目标是利用表面 SERR对确定结构信息的敏感性 蛋白质。 第四个目标是追求纯粹的分析性应用 SERS/SERRS。这将包括检测由 层析法。一种检测小程序交互的方法 具有捕获蛋白的发色分子也将被 调查过了。在上述所有研究中，将努力做到 通过提高表面拉曼技术的灵敏度 使用新的实验方法(即光纤， 衰减全反射和微拉曼采样 程序)。探测器技术的进步也将是 对于这一目标很重要。