LASER RAMAN SPECTROMETER

请求提供资金，以建立一个多用户拉曼设施， 内布拉斯加大学林肯分校的化学系。 该设施将包含仪器， 拉曼光谱学领域的前峰。 这将包括 Nd：YAG泵浦的脉冲染料激光器和波长扩展器， 提供从216 nm到近红外的波长可调谐性。 共振拉曼光谱的最大灵敏度需要可调谐性 实验 所要求的光谱仪被选择来提供 尽可能广泛的研究能力。 开发 Instruments S.A.，宏观/微观分光光度计具有 单通道和多通道检测能力。 专用 计算机将与系统一起购买，其中包括所有 多任务操作和数据分析软件。 的 仪器可以很容易地从一个采样切换到 从一个配置到另一个配置（例如，微观到宏观采样）。 该系统的另一个重要优点是， 设施是其操作简单。 由于大多数操作 计算机控制，研究生和教师将很快 获得必要的技能来记录光谱。 安全功能 防止敏感探测器损坏 由于过度暴露于光而导致的。 拉曼设施将有五个主要用户。 这些将 包括首席研究员，一名拉曼光谱学家，三名 额外的NIH资助的教师和一名教师谁是 目前没有得到NIH的资助。 的研究兴趣 主要研究者关注的是 表面增强拉曼散射技术研究 生物分子 长期目标是利用这些技术， 在原位研究生物分子。 另一方的研究兴趣 研究人员包括对激光解吸过程的研究， 在生物分子的质谱，蛋白质-DNA相互作用， 用于新合成方法的活性金属粉末和金属- 核苷酸相互作用 可用的结构信息 从拉曼、共振拉曼和表面增强拉曼 方法论将有益于正在进行的研究 这些调查人员的努力。 拉曼光谱学是理想的 适用于各种类型的样品， 研究中的问题。 预计额外的教师， 无论是在化学和其他部门在UNL，也将使用 工具。

Funds are requested to establish a multi-user Raman facility in the Chemistry Department at the University of Nebraska-Lincoln. The facility would contain instrumentation which is at the forefron in the field of Raman spectroscopy. This would include a Nd:YAG pumped, pulsed dye laser and wavelength extender, which provides wavelength tunability from 216 nm to the near infrared. Tunability is needed for maximum sensitivity in resonance Raman experiments. The spectrometer requested was chosen to provide the broadest possible research capabiflities. Developed by Instruments S.A., the macro/microspectrophotomer has both single and multichannel detection capabilities. A dedicated computer will be purchased with the system, which includes all of the software for multitasking operations and data analysis. The instrument can easily be switched from one sampling configuration to another (e.g. micro- to macro-sampling). Another important advantage of this system for a multiple user facility is its simplicity of operation. Since most operations are computer controlled, graduate students and faculty will quickly acquire the necessary skills to record spectra. Safety features are included which prevent damage of the sensitive dectors resulting from overexposure to light. There will be five major users of the Raman facility. These will include the Principle Investigator, a Raman spectroscopist, three additional NIH-funded faculty and one faculty member who is currently not funded by NIH. The research interests of the Principle Investigator are concerned with the development of surface enhanced Raman scattering techniques for the study of biomolecules. The long term goal is to utilize these techniques to study biomolecules in situ. The research interests of the other investigators include the study of laser desorption processes used in mass spectrometry of biomolecules, protein-DNA interactions, active metal powders for new synthetic methods, and metal- nucleotide interactions. The structural information available from Raman, resonance Raman and surface enhanced Raman methodology will benefit considerabley the on-going research efforts of these investigators. Raman spectroscopy is ideally suited for application to the diverse types of samples and problems under study. It is anticipated that additional faculty, both in Chemistry and other Departments at UNL, will also use the instruments.