DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
批准号:
3289671
负责人:
Charles J Waechter
金额:
$15.8万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1981
资助国家:
美国
项目状态:
已结题
起止时间:
1981-06-01 至 1992-05-31
中文摘要
活化的b淋巴细胞(b细胞)导致增加
英文摘要
The activation of B-lymphocytes (B-cells) leads to increased
synthesis of membrane-bound and secretory glycoproteins. This
application proposes studies on the regulation of N-glycosylation
activity when membrane glycoprotein synthesis is stimulated in
resting B-cells induced to proliferate by bacterial
lipopolysaccharide (LPS). The control of N-glycosylation will also
be investigated in the human B lymphoblastoid cell line (CESS)
stimulated to differentiate and secrete large amounts of
immunoglobulins by exposure to B-cell differentiation factor
(BCDF). Changes in N-glycosylation activity in response to the
induction of proliferation or differentiation of the B-cells will be
correlated with changes in several enzymes involved in dolichyl
phosphate (Do1-P) metabolism and the synthesis of dolichol-linked
oligosaccharides. Dolichol kinase and Dol-P phosphatase activity
will be assayed in vitro to determine if Dol-P levels could be
altered by shifting the balance in a phosphorylation-
dephosphorylation scheme during the proliferation of B-cells or
the differentiation of the CESS line. The requirement for de novo
synthesis of dolichol or Dol-P during the proliferative and
differentiative changes will be explored by metabolic labeling
experiments with (3H)mevalonate, and studies with exogenously
supplied dolichol and capactin, the competitive inhibitor of HMG-
CoA reductase. The possible involvement of Ca++-mobilization
and protein kinase C in the activation processes will be explored
by cellular studies with ionomycin and phorbol diesters. The
proposed experiments could shed new light on the control of the
N-glycosylation apparatus in B-cells, and extend the current
knowledge of the biochemical events occurring during the
proliferation and differentiation of B-lymphocytes.
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会议论文
In Search of Mannolipid Intermediate Flippases
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批准号:8710272
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项目类别:
-
资助金额:$28.22万
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财政年份:2012
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负责人:Charles J Waechter
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依托单位:
In Search of Mannolipid Intermediate Flippases
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批准号:8341035
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项目类别:
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资助金额:$28.22万
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财政年份:2012
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负责人:Charles J Waechter
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依托单位:
In Search of Mannolipid Intermediate Flippases
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批准号:8548379
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项目类别:
-
资助金额:$27.23万
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财政年份:2012
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负责人:Charles J Waechter
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依托单位:
Regulation of Protein N-Glycosylation in the CNS
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批准号:7923610
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项目类别:
-
资助金额:$11.26万
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财政年份:2009
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负责人:Charles J Waechter
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依托单位:
MUTAGENESIS OF N-GLYCOSYLATION SITES IN CHOLINESTERASE
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批准号:3023496
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项目类别:
-
资助金额:$2.08万
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财政年份:1993
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负责人:Charles J Waechter
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依托单位:
DOLICHOL METABOLISM AND MEMBRANE GLYCOPROTEIN BIOSYNTHES
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批准号:3289668
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项目类别:
-
资助金额:$6.6万
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财政年份:1985
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负责人:Charles J Waechter
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依托单位:
DOLICHOL METABOLISM AND MEMBRANE GLYCOPROTEIN BIOSYNTHES
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批准号:3289674
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项目类别:
-
资助金额:$15.3万
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财政年份:1985
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负责人:Charles J Waechter
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依托单位:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
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批准号:2178206
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项目类别:
-
资助金额:$21.82万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
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批准号:3289666
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项目类别:
-
资助金额:$15.39万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
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批准号:3289667
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项目类别:
-
资助金额:$20.64万
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财政年份:1981
-
负责人:Charles J Waechter
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依托单位:
REGULATION OF PROTEIN N-GLYCOSYLATION IN THE CNS
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批准号:6525577
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项目类别:
-
资助金额:$31.86万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
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批准号:2178205
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项目类别:
-
资助金额:$21.49万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
-
批准号:3289673
-
项目类别:
-
资助金额:$20.56万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
-
批准号:3289672
-
项目类别:
-
资助金额:$15.93万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
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批准号:3289669
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项目类别:
-
资助金额:$14.22万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
REGULATION OF PROTEIN N-GLYCOSYLATION IN THE CNS
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批准号:6656974
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项目类别:
-
资助金额:$31.86万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
Regulation of Protein N-Glycosylation in the CNS
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批准号:7492043
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项目类别:
-
资助金额:$31.25万
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财政年份:1981
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负责人:Charles J Waechter
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依托单位:
REGULATION OF PROTEIN N GLYCOSYLATION IN THE CNS
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批准号:2178207
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项目类别:
-
资助金额:$22.38万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
Regulation of Protein N-Glycosylation in the CNS
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批准号:7282500
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项目类别:
-
资助金额:$31.25万
-
财政年份:1981
-
负责人:Charles J Waechter
-
依托单位:
REGULATION OF PROTEIN N GLYCOSYLATION IN THE CNS
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批准号:2546016
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项目类别:
-
资助金额:$23.27万
-
财政年份:1981
-
负责人:Charles J Waechter
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依托单位:
海外基金