DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
磷酸多醇代谢和糖蛋白合成
基本信息
- 批准号:3289666
- 负责人:
- 金额:$ 15.39万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1981
- 资助国家:美国
- 起止时间:1981-06-01 至 1992-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The activation of B-lymphocytes (B-cells) leads to increased
synthesis of membrane-bound and secretory glycoproteins. This
application proposes studies on the regulation of N-glycosylation
activity when membrane glycoprotein synthesis is stimulated in
resting B-cells induced to proliferate by bacterial
lipopolysaccharide (LPS). The control of N-glycosylation will also
be investigated in the human B lymphoblastoid cell line (CESS)
stimulated to differentiate and secrete large amounts of
immunoglobulins by exposure to B-cell differentiation factor
(BCDF). Changes in N-glycosylation activity in response to the
induction of proliferation or differentiation of the B-cells will be
correlated with changes in several enzymes involved in dolichyl
phosphate (Do1-P) metabolism and the synthesis of dolichol-linked
oligosaccharides. Dolichol kinase and Dol-P phosphatase activity
will be assayed in vitro to determine if Dol-P levels could be
altered by shifting the balance in a phosphorylation-
dephosphorylation scheme during the proliferation of B-cells or
the differentiation of the CESS line. The requirement for de novo
synthesis of dolichol or Dol-P during the proliferative and
differentiative changes will be explored by metabolic labeling
experiments with (3H)mevalonate, and studies with exogenously
supplied dolichol and capactin, the competitive inhibitor of HMG-
CoA reductase. The possible involvement of Ca++-mobilization
and protein kinase C in the activation processes will be explored
by cellular studies with ionomycin and phorbol diesters. The
proposed experiments could shed new light on the control of the
N-glycosylation apparatus in B-cells, and extend the current
knowledge of the biochemical events occurring during the
proliferation and differentiation of B-lymphocytes.
B淋巴细胞(B细胞)的活化导致增加的
膜结合和分泌糖蛋白的合成。 这
应用提出了对N-糖基化的调节的研究
当膜糖蛋白合成被刺激时,
细菌诱导的静息B细胞增殖
脂多糖(LPS)。 N-糖基化的控制也将
在人B淋巴母细胞样细胞系(CESS)中进行研究
刺激分化并分泌大量的
B细胞分化因子免疫球蛋白
(BCDF)。 N-糖基化活性的变化,
B细胞增殖或分化的诱导将是
与参与长叶醇合成的几种酶的变化相关
磷酸盐(Do 1-P)代谢和合成多萜醇连接的
低聚糖。 Dolichol激酶和Dol-P磷酸酶活性
将在体外进行测定,以确定是否可以
通过改变磷酸化平衡而改变
B细胞增殖过程中的去磷酸化方案,或
CESS系列的差异化。 重新分类的要求
在增殖过程中合成多萜醇或Dol-P,
将通过代谢标记来探索分化变化
(3 H)甲羟戊酸的实验,以及外源性
提供了多萜醇和capactin,HMG的竞争性抑制剂,
CoA还原酶。 Ca++动员的可能参与
和蛋白激酶C在激活过程中的作用
通过离子霉素和佛波醇二酯的细胞研究。 的
拟议的实验可以揭示新的光控制的
在B细胞中的N-糖基化装置,并延长电流
生物化学过程中发生的事件的知识
B淋巴细胞的增殖和分化。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Charles J Waechter其他文献
Charles J Waechter的其他文献
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{{ truncateString('Charles J Waechter', 18)}}的其他基金
Regulation of Protein N-Glycosylation in the CNS
CNS 中蛋白质 N-糖基化的调节
- 批准号:
7923610 - 财政年份:2009
- 资助金额:
$ 15.39万 - 项目类别:
MUTAGENESIS OF N-GLYCOSYLATION SITES IN CHOLINESTERASE
胆碱酯酶中 N-糖基化位点的诱变
- 批准号:
3023496 - 财政年份:1993
- 资助金额:
$ 15.39万 - 项目类别:
DOLICHOL METABOLISM AND MEMBRANE GLYCOPROTEIN BIOSYNTHES
多醇代谢和膜糖蛋白生物合成
- 批准号:
3289668 - 财政年份:1985
- 资助金额:
$ 15.39万 - 项目类别:
DOLICHOL METABOLISM AND MEMBRANE GLYCOPROTEIN BIOSYNTHES
多醇代谢和膜糖蛋白生物合成
- 批准号:
3289674 - 财政年份:1985
- 资助金额:
$ 15.39万 - 项目类别:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
磷酸多醇代谢和糖蛋白合成
- 批准号:
2178206 - 财政年份:1981
- 资助金额:
$ 15.39万 - 项目类别:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
磷酸多醇代谢和糖蛋白合成
- 批准号:
3289671 - 财政年份:1981
- 资助金额:
$ 15.39万 - 项目类别:
DOLICHOL PHOSPHATE METABOLISM AND GLYCOPROTEIN SYNTHESIS
磷酸多醇代谢和糖蛋白合成
- 批准号:
3289667 - 财政年份:1981
- 资助金额:
$ 15.39万 - 项目类别:
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