DOLICHOL METABOLISM AND MEMBRANE GLYCOPROTEIN BIOSYNTHES

多醇代谢和膜糖蛋白生物合成

• 批准号: 3289674
• 负责人: Charles J Waechter
• 金额: $ 15.3万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 1987-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3289674
• 关键词: animal age group; brain; brain metabolism; carbohydrate biosynthesis; carbohydrate structure; central nervous system; dolichol; embryo /fetus; enzyme structure; glycolipids; glycosyltransferase; growth /development; heparan sulfate; lipase; lipid metabolism; microsomes; neurochemistry; neurotransmitter metabolism; peptidases; phosphoric ester; radiotracer; sulfotransferase; tissue /cell culture

Studies with calf brain microsomes and tissue slices on the den novo synthesis of dolichyl monophosphate are proposed. Extensive work on the purification of dolichol kinase, dolichyl monophosphatase and dolichyl ester lipase will be done to establish their properties and specificity. The possibility that changes in these activities influence the level of dolichyl monophosphate during development in the central nervous system will be explored. In addition the subcellular location(s) of the three enzymes mentioned above and the various lipid-mediated glycosyltransferases in brain will be studied. We shall also attempt to purify sulfotransferases involved in the biosynthesis of a glycoprotein and proteoheparan sulfate associated with calf brain membranes. The structural requirements and the properties of the binding interaction between the proteoheparan sulfate and calf membranes will be investigated.

用小牛脑微粒体和组织切片对新生儿的研究 提出了一种新合成方法。 关于《公约》的广泛工作 多萜醇激酶、多萜醇单磷酸酶和多萜醇的纯化 将酯脂肪酶做了研究，以确定其性质和特异性。 这些活动的变化可能会影响 中枢神经系统发育过程中的一磷酸长叶醇 将被探索。 此外，这三种蛋白的亚细胞位置 上述酶和各种脂质介导的糖基转移酶 大脑将被研究。 我们还将尝试净化 参与糖蛋白生物合成的磺基转移酶， 与小牛脑膜相关的硫酸蛋白乙酰肝素。 结构性 之间的绑定交互的要求和属性， 将研究硫酸蛋白乙酰肝素和小牛膜。