LOW MOLECULAR WEIGHT SUPEROXIDE DISMUTASE IN HEART

心脏中的低分子量超氧化物歧化酶

• 批准号: 3295059
• 负责人: EUGENE M GREGORY
• 金额: $ 7.73万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3295059
• 关键词: azides; copper; cyanides; electron spin resonance spectroscopy; enzyme substrate; gel filtration chromatography; heart metabolism; immunochemistry; ion exchange chromatography; ligands; lung; manganese; mass spectrometry; metalloenzyme; molecular weight; stoichiometry; superoxide dismutase; ultracentrifugation

Organisms cope with the oxidative stress of an oxygen environment by synthesizing antioxidant enzymes. These enzymes are usually 20,000 to 100,000 molecular weight and have either transition metals or heme as catalytic components. Superoxide dismutases scavenge the superoxide radical and protect the cell from the deleterious effects of that radical. A novel superoxide dismutase activity has recently been found in extracts of heart and lung tissue. The activity, named ersatz SOD, is associated with a heat stable, low molecular weight ligand specific for Cu or Mn. The general goal of the research is to characterize the ligand components both physically and chemically. The component(s) will be isolated to homogeneity by ultrafiltration, gel exclusion and ion exchange chromatography. Optimal conditions for assay of the catalytic activity both spectrophotometrically and histochemically will be developed. Composition of the ersatz SOD will be determined by amino acid analysis and Fast Atom Bombardment Mass Spectrometry. The uv-visible and electron paramagnetic resonance spectra of the hololigand and metalloligand complex will be measured. Location of the ligand within heart and lung tissue will be found with immunocytochemical techniques. There is the potential for use of the SOD mimic as a therapeutic or anaphalytic agent during medically imposed ischemia - reperfusion and as a possible radioprotectant during radiation therapy.

生物体科普氧气的氧化应激 环境中合成抗氧化酶。 这些酶 分子量通常为20，000至100，000， 过渡金属或血红素作为催化组分。 超氧 歧化酶抑制超氧自由基，保护细胞 免受这种自由基的有害影响。 一种新型超氧化物 最近在心脏提取物中发现了歧化酶活性， 和肺组织。 这种名为ErbD-SOD的活性与 具有热稳定的、对Cu特异的低分子量配体，或 MN. 研究的总目标是表征配体 物理和化学成分。 组件 将通过超滤、凝胶排阻分离至均匀 和离子交换色谱法。 最佳含量测定条件 的催化活性， 组织化学将被开发。 厄立特里亚的组成 SOD将通过氨基酸分析和快速原子吸收法测定 轰击质谱法。 紫外可见光和电子 全配体的顺磁共振谱， 将测量金属配体络合物。 配体的位置 在心脏和肺组织中会发现 免疫细胞化学技术。 SOD模拟物具有作为治疗药物的潜力 或在医学上施加的缺血期间的促炎剂- 再灌注和作为辐射期间可能的辐射防护剂 疗法