SUPEROXIDE DISMUTASE & CATALASE IN B FRAGILIS

厌氧菌在短暂通过后可能会形成软组织感染部位 空气，或通过比正常情况下更易氧化的环境 栖息地。尽管关于氧气的各种影响的信息很少， 超氧化物歧化酶、过氧化氢酶等抗氧化物酶和营养素 来源取决于需氧菌或耐氧物种产生柔软的 组织感染，这种信息对于厌氧菌来说几乎是不存在的。这个 建议的研究目的是确定细胞内的影响 以及细胞间超氧化物歧化酶和过氧化氢酶水平对能力的影响 脆弱类杆菌和其他厌氧菌抵抗氧气依赖 破坏吞噬细胞，形成实验性感染部位，以及 能承受高压氧和氧代谢物应激。脆弱假单胞菌含有 一种O2诱导的超氧化物歧化酶。增加了这种酶的水平 对高压氧胁迫的抵抗力。感受性和感受性 未诱导的细胞吞噬杀伤和它们在体内产生脓肿的能力 SITE将被关联。过氧化氢酶水平可以通过改变两者来操纵 脆弱芽孢杆菌培养过程中的葡萄糖和氯化血红素水平。已更改的细胞 过氧化氢酶水平也将受到这里描述的测试系统的影响。 这些研究旨在寻求对以下问题的量化答案 空气耐受性和感染过程。

Anaerobes may develop soft tissue infection sites after a brief passage through air, or through a more oxidizing environment than they encounter in their normal habitat. Although little information concerning the sundry effects of oxygen, antioxidant enzymes such as superoxide dismutase and catalase, and nutrient sources upon the ability of aerobes or aerotolerant species to generate a soft tissue infection, that information is virtually nonexistant for anaerobes. The purpose of the proposed research is to determine the effects of intracellular and intercellular levels of superoxide dismutase and catalase upon the ability of Bacteroides fragilis and other anaerobes to resist oxygen dependent phagocytic cell destruction, develop an experimental infection site, and withstand hyperbaric oxygen and oxygen metabolite stress. B. fragilis contains an O2-inducible superoxide dismutase. Increased levels of that enzyme impart resistance to hyperbaric oxygen stress. The susceptibility of induced and uninduced cells to phagocytic kill and their ability to generate an abscess in situ will be correlated. Catalase levels may be manipulated by varying both glucose and hemin levels during culture of B. fragilis. Cells with altered catalase levels will also be subjected to the test systems described herein. These studies are designed to seek quantitative answers to questions concerning aerotolerance and the infection process.