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SUPEROXIDE DISMUTASE & CATALASE IN B FRAGILIS

超氧化物歧化酶

基本信息

批准号：
3126101
负责人：
EUGENE M GREGORY
金额：
$ 8.03万
依托单位：
VIRGINIA POLYTECHNIC INST AND ST UNIV
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
起止时间：
1978-08-01 至 1990-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3126101
关键词：
Bacteroides anaerobic bacteria catalase free radicals phagocytosis superoxides

项目摘要

Anaerobes may develop soft tissue infection sites after a brief passage through air, or through a more oxidizing environment than they encounter in their normal habitat. Although little information concerning the sundry effects of oxygen, antioxidant enzymes such as superoxide dismutase and catalase, and nutrient sources upon the ability of aerobes or aerotolerant species to generate a soft tissue infection, that information is virtually nonexistant for anaerobes. The purpose of the proposed research is to determine the effects of intracellular and intercellular levels of superoxide dismutase and catalase upon the ability of Bacteroides fragilis and other anaerobes to resist oxygen dependent phagocytic cell destruction, develop an experimental infection site, and withstand hyperbaric oxygen and oxygen metabolite stress. B. fragilis contains an O2-inducible superoxide dismutase. Increased levels of that enzyme impart resistance to hyperbaric oxygen stress. The susceptibility of induced and uninduced cells to phagocytic kill and their ability to generate an abscess in situ will be correlated. Catalase levels may be manipulated by varying both glucose and hemin levels during culture of B. fragilis. Cells with altered catalase levels will also be subjected to the test systems described herein. These studies are designed to seek quantitative answers to questions concerning aerotolerance and the infection process.

厌氧菌在短暂通过后可能会形成软组织感染部位空气中，或通过比他们在正常情况下遇到的氧化环境，栖息地尽管关于氧气的生物效应的资料很少，抗氧化酶如超氧化物歧化酶和过氧化氢酶，以及营养素需氧菌或耐氧菌物种产生柔软的组织感染，这一信息实际上是不存在的厌氧菌。的这项研究的目的是确定细胞内和细胞间水平的超氧化物歧化酶和过氧化氢酶的能力脆弱拟杆菌和其他厌氧菌的抗氧依赖性吞噬细胞破坏，形成实验感染部位，以及承受高压氧和氧代谢物的压力。 B。 fragilis包含一种O2诱导型超氧化物歧化酶。这种酶水平的增加抗高压氧应激。诱导和未诱导的细胞吞噬杀死和他们的能力，产生脓肿，现场将是相关的。过氧化氢酶水平可以通过改变 B培养过程中的葡萄糖和氯高铁血红素水平。脆弱的改变的细胞过氧化氢酶水平也将经受本文所述的测试系统。这些研究旨在寻求定量答案的问题，耐氧性和感染过程。