A brighter future cutting-edge multiphoton imaging at Nottingham

诺丁汉尖端多光子成像的光明未来

• 批准号: BB/X019241/1
• 负责人: Kim Chisholm
• 金额: $ 170.23万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FX019241%2F1
• 关键词: brighter; future; cutting; edge; multiphoton

Optical microscopes provide a detailed view into the fundamental processes of life: they let us look at what is happening inside cells, or between different biological networks. Through the development of minimally invasive imaging techniques, we can now do so in intact tissues or even intact whole organisms. The multiphoton microscope is the go-to instrument to be able to achieve such three-dimensional investigations of live tissues. The multiphoton microscope can visualise common fluorescent signals and tissue structures through much longer and less toxic, invisible wavelengths, than would be used on more conventional microscopes. This provides a host of advantages. Firstly, it means that we can observe biological processes with less effect on the system we are studying - this is because molecules present naturally in biological tissues are less likely to be damaged by these longer wavelengths. Secondly, the longer wavelengths used in multiphoton microscopy enable us to look deeper into tissues because longer wavelengths interact less with biological tissues. Thirdly this technique allows us to resolve signals in three-dimensions, including depth, allowing us to differentiate structures that are close to one another, even when one is on top of the other. These combined abilities, currently not available in Nottingham, open a range of opportunities to investigate important biological processes which are fundamental to the rules of life and health. For example, this multiphoton microscope will enable us to image thick and complex tissues or biological materials that are being developed in Nottingham, in 3D. We will be able to visualise processes occurring in real-time, in the whole animal which is important because many very intricate networks (e.g. nervous system and vascular networks) cannot easily be reproduced in a dish. This platform will also integrate with already existing expertise and workflows in Nottingham, to look at the same samples from many different angles and scales. This enables translational, multimodal research that is not possible anywhere else in the country. With this system, researchers in Nottingham and regional industrial partners, will be able to better understand these complex tissues and systems, and study changes occurring in real-time, with better clarity and minimal effect on the systems being studied.This multiphoton microscope is an essential tool missing in the research capabilities of academic and industrial networks in Nottingham. It will help attract and retain talented researchers in the Midlands by supporting existing research on the fundamentals of life and health and provide new essential capabilities to the region with yet untapped potential.

光学显微镜为生活的基本过程提供了详细的看法：它们让我们看看细胞内部发生的事情，或者在不同的生物网络之间发生了什么。通过开发微创成像技术，我们现在可以在完整的组织甚至完整的整个生物体中这样做。多光子显微镜是能够实现对活组织的三维研究的首选工具。多光子显微镜可以通过更长，毒性更少的无形波长来可视化常见的荧光信号和组织结构，而不是在更常规的显微镜上使用。这提供了许多优势。首先，这意味着我们可以观察到对我们正在研究的系统影响较小的生物学过程 - 这是因为在生物组织中天然存在的分子不太可​​能被这些较长的波长损坏。其次，多光子显微镜中使用的较长波长使我们能够更深入地研究组织，因为较长的波长与生物组织相互作用较少。第三，这项技术使我们能够以三维（包括深度）来解析信号，从而使我们能够区分彼此接近的结构，即使一个结构在另一个结构上也是在另一个方面。这些合并能力目前在诺丁汉，为研究重要的生物学过程提供了一系列机会，这些过程对生活和健康规则至关重要。例如，该多光子显微镜将使我们能够在3D中成像诺丁汉开发的厚而复杂的组织或生物材料。在整个动物中，我们将能够实时可视化发生的过程，这很重要，因为许多非常复杂的网络（例如神经系统和血管网络）无法轻易在菜肴中复制。该平台还将与诺丁汉已经存在的专业知识和工作流程集成，以从许多不同的角度和尺度上查看相同的样本。这使得在该国其他任何地方都不可能进行翻译，多模式研究。借助该系统，诺丁汉和区域工业伙伴的研究人员将能够更好地理解这些复杂的组织和系统，并实时研究变化，对所研究系统的更好的清晰度和最小的影响，这是多功能显微镜是诺丁汉的学术和工业网络研究能力中缺少的重要工具。它将通过支持现有关于生活和健康基础的研究，并为该地区提供尚未开发的潜力的新基本能力，从而吸引和留住中部地区的才华横溢的研究人员。