A brighter future cutting-edge multiphoton imaging at Nottingham

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Optical microscopes provide a detailed view into the fundamental processes of life: they let us look at what is happening inside cells, or between different biological networks. Through the development of minimally invasive imaging techniques, we can now do so in intact tissues or even intact whole organisms. The multiphoton microscope is the go-to instrument to be able to achieve such three-dimensional investigations of live tissues. The multiphoton microscope can visualise common fluorescent signals and tissue structures through much longer and less toxic, invisible wavelengths, than would be used on more conventional microscopes. This provides a host of advantages. Firstly, it means that we can observe biological processes with less effect on the system we are studying - this is because molecules present naturally in biological tissues are less likely to be damaged by these longer wavelengths. Secondly, the longer wavelengths used in multiphoton microscopy enable us to look deeper into tissues because longer wavelengths interact less with biological tissues. Thirdly this technique allows us to resolve signals in three-dimensions, including depth, allowing us to differentiate structures that are close to one another, even when one is on top of the other. These combined abilities, currently not available in Nottingham, open a range of opportunities to investigate important biological processes which are fundamental to the rules of life and health. For example, this multiphoton microscope will enable us to image thick and complex tissues or biological materials that are being developed in Nottingham, in 3D. We will be able to visualise processes occurring in real-time, in the whole animal which is important because many very intricate networks (e.g. nervous system and vascular networks) cannot easily be reproduced in a dish. This platform will also integrate with already existing expertise and workflows in Nottingham, to look at the same samples from many different angles and scales. This enables translational, multimodal research that is not possible anywhere else in the country. With this system, researchers in Nottingham and regional industrial partners, will be able to better understand these complex tissues and systems, and study changes occurring in real-time, with better clarity and minimal effect on the systems being studied.This multiphoton microscope is an essential tool missing in the research capabilities of academic and industrial networks in Nottingham. It will help attract and retain talented researchers in the Midlands by supporting existing research on the fundamentals of life and health and provide new essential capabilities to the region with yet untapped potential.