ENZYMES OF AROMATIC ACID METABOLISM

芳香酸代谢酶

• 批准号: 3292370
• 负责人: John W Kozarich
• 金额: $ 10.69万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3292370
• 关键词: Fungi Imperfecti; Pseudomonas; catechols; cyclic acid; enol; enzyme substrate; enzymes; halocarbon compound; hydrolase; isomerase; lactones; nuclear magnetic resonance spectroscopy; stereochemistry; ultraviolet spectrometry

Our long term goals in this research is to thoroughly investigate the rich mechanistic and stereochemical questions associated with the enzymes of aromatic acid matabolism. These enzymes, particularly those constituting the Beta-ketoadipate pathway, are of considerable interest from an intellectual as well as practical standpoint. These studies should shed light on the role of mechanism and stereochemistry in the evolution of these enzymes as they adapted to specific functions and specific substrates. In addition, the biodegradation of aromatics is becoming increasingly important as a potential solution to the problem of polution control. Our specific aims during the funding period will be: (1) A study of the mechanism and stereochemistry of the enzymes of the Beta-ketoadipate pathway from P. putida. (2) The analysis of the reactions of haloprotocatechuates with the P. putida enzymes. (3) A study of the mechanism and stereochemistry of enzymes of A. calcoaceticus, particulary the two enol-lactone hydrolases. (4) An analysis of the Pseudomonas sp B13 enzymes, particularly the specific enzymes responsible halocatechol metabolism. (5) A study of the enzymes from the eukaryote, Trichosporon cutaneum, which appear to have different specificities. We believe that these basic studies should serve as a foundation for the genetic manipulation of microorganisms to meet specific detoxification needs. Furthermore, the studies will increase our mechanistic understanding of these intriguing enzymes.

我们这项研究的长期目标是彻底调查富人 与酶相关的机械和立体化学问题 芳香酸代谢。 这些酶，特别是那些构成 Beta-酮己二酸途径，引起了人们的极大兴趣 智力和实践的立场。 这些研究应该揭示 阐明机制和立体化学在进化中的作用 这些酶适应特定的功能和特定的 基材。 此外，芳烃的生物降解正在变得越来越重要。 作为污染问题的潜在解决方案越来越重要 控制。 我们在资助期间的具体目标是： (1) 酶的作用机理和立体化学研究 来自恶臭假单胞菌的β-酮己二酸途径。 (2)卤代儿茶酸酯与P.的反应分析。 恶臭酶。 (3)A.酶的作用机理和立体化学研究。 calcoacetus，特别是两种烯醇内酯水解酶。 (4) 假单胞菌 B13 酶的分析，特别是 负责卤代儿茶酚代谢的特定酶。 (5) 对真核生物皮肤毛孢子酶的研究， 似乎有不同的特殊性。 我们认为，这些基础研究应该作为 对微生物进行基因操作以满足特定的解毒作用 需要。 此外，这些研究将增加我们的机制 了解这些有趣的酶。