A multiphoton confocal microscope for deep tissue imaging in live organisms

我们是伦敦国王学院的一组科学家，他们希望购买一种专门的显微镜，可以对生物体中的微小结构进行成像，例如血管壁或神经突起。这种特殊的显微镜被特别改装，使研究人员能够分析组织内部发生的事件，而这些事件通常无法使用正常的研究显微镜进行访问。它还可以防止我们使用的成像方案造成的任何损害，因此我们可以可视化细胞和组织的行为，而不会影响它们的功能。我们将使用这种显微镜在生物科学的许多不同领域进行研究，包括免疫学、组织学和神经科学。我们将能够对器官内的单个细胞进行成像，分析它们与其他细胞和隔间的相互作用，并确定这些细胞对创伤或病原体的反应。我们的结果将帮助我们更多地了解我们的身体是如何工作的，我们如何对抗正常的日常感染，以及我们的细胞和组织在衰老过程中如何变化。显微镜将被放置在一个专门建造的设施中，那里有训练有素、敬业的工作人员来照顾它。他们将培训其他人在未来如何使用它来解决自己的研究问题。这意味着，该仪器不仅将使我们的团队受益，也将使我们大学内外的其他科学家受益。

We are a team of scientists at King's College London who wish to buy a specialist microscope that can image tiny structures in living organisms, such as blood vessel walls or nerve processes. This particular microscope is specially adapted to allow researchers to analyse events that occur deep within tissues, which are usually not accessible using normal research microscopes. It is also adapted to prevent any damage occurring from the imaging protocols we use, so we can visualise cell and tissue behaviour without affecting their function. We would use this microscope to conduct research in many different areas of biological science, including immunology, tissue organisation and neuroscience. We will be able to image individual cells within organs, analyse their interactions with other cells and compartments and determine how these cells respond to wounds or pathogens. Our results will help us understand more about how our bodies work, how we combat normal everyday infections and how our cells and tissues change during ageing. The microscope would be placed within a purpose-built facility that has highly trained, dedicated staff to look after it. They will train other people how to use it in future for their own research questions. This means that the instrument would not only benefit our team, but also other scientists at our university and beyond.