A multiphoton confocal microscope for deep tissue imaging in live organisms

用于活体深层组织成像的多光子共焦显微镜

• 批准号: BB/X019861/1
• 负责人: Franziska Denk
• 金额: $ 78.44万
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FX019861%2F1
• 关键词: multiphoton; confocal; microscope; deep; tissue

We are a team of scientists at King's College London who wish to buy a specialist microscope that can image tiny structures in living organisms, such as blood vessel walls or nerve processes. This particular microscope is specially adapted to allow researchers to analyse events that occur deep within tissues, which are usually not accessible using normal research microscopes. It is also adapted to prevent any damage occurring from the imaging protocols we use, so we can visualise cell and tissue behaviour without affecting their function. We would use this microscope to conduct research in many different areas of biological science, including immunology, tissue organisation and neuroscience. We will be able to image individual cells within organs, analyse their interactions with other cells and compartments and determine how these cells respond to wounds or pathogens. Our results will help us understand more about how our bodies work, how we combat normal everyday infections and how our cells and tissues change during ageing. The microscope would be placed within a purpose-built facility that has highly trained, dedicated staff to look after it. They will train other people how to use it in future for their own research questions. This means that the instrument would not only benefit our team, but also other scientists at our university and beyond.

我们是伦敦国王学院的一个科学家团队，他们希望购买一台专业显微镜，可以对生物体中的微小结构（例如血管壁或神经过程）进行成像。这种特殊的显微镜经过专门设计，可以让研究人员分析组织深处发生的事件，而使用普通研究显微镜通常无法观察到这些事件。它还适用于防止我们使用的成像协议造成任何损害，因此我们可以可视化细胞和组织的行为而不影响其功能。我们将使用这款显微镜在生物科学的许多不同领域进行研究，包括免疫学、组织组织和神经科学。我们将能够对器官内的单个细胞进行成像，分析它们与其他细胞和区室的相互作用，并确定这些细胞如何对伤口或病原体做出反应。我们的结果将帮助我们更多地了解我们的身体如何运作、如何对抗正常的日常感染以及我们的细胞和组织在衰老过程中如何变化。显微镜将被放置在一个专门建造的设施内，该设施有训练有素的专门人员来照顾它。他们将培训其他人如何在未来使用它来解决自己的研究问题。这意味着该仪器不仅将使我们的团队受益，而且还会使我们大学内外的其他科学家受益。