Multiphoton confocal microscope

为了表征多细胞结构，显微镜技术需要确保高空间和时间分辨率，甚至在材料内部几百微米深。这是高度相关的，特别是在组织工程的背景下，以及在器官型3D组织培养系统。此外，3d结构材料对于研究细胞生长非常有趣。这种生物混合材料细胞系统的微观表征对于理解不同材料对三维细胞行为的影响是绝对必要的。因此，需要高穿透深度和低细胞毒性的多光子共聚焦显微镜来开展高空间分辨率和长时间尺度的三维活细胞系统研究项目。该系统应在若干波长范围内工作，以确保在若干项目和合作中广泛适用。此外，它必须允许在三维结构中研究细胞生长的长期测量。显微镜系统的一个重要应用是研究由双光子光刻或生物打印方法产生的三维材料特性对细胞、多细胞结构和组织结构的影响。

For characterizing multicellular structures, microscopy techniques are required that ensure high spatial and temporal resolution even several hundred micrometers deep inside a material. This is highly relevant especially in the context of tissue engineering as well as in organotypic 3D tissue culture systems. Furthermore, 3D-structured materials are highly interesting to study cell growth. The microscopic characterization of such biohybrid material cell systems is absolutely necessary to understand the impact of different materials on cell behavior in 3D. Therefore, a multiphoton confocal microscope with high penetration depth and low cytotoxicity is required to carry out projects in which living cellular systems are investigated in three dimensions with high spatial resolution and over longer timescales. The system should operate in several wavelength ranges to ensure broad applicability in several projects and collaborations. In addition, it must allow long-term measurements to study the growth of cells in three-dimensional structures. An important application of the microscopy system is the investigation of the influence of three-dimensional material properties, generated by two-photon lithography or bio-printing methods, on cells, multicellular structures and tissue structures.