Multiphoton confocal microscope

为了表征多细胞结构，需要显微技术来确保材料内部甚至数百微米深处的高空间和时间分辨率。这是高度相关的，特别是在组织工程的背景下以及在器官型3D组织培养系统中。此外，三维结构材料在研究细胞生长方面具有很高的兴趣。为了了解不同材料对细胞三维行为的影响，对这种生物杂化材料细胞系统的微观表征是绝对必要的。因此，需要一种高穿透深度、低细胞毒性的多光子共聚焦显微镜，以高空间分辨率和更长的时间尺度对活细胞系统进行三维研究。该系统应该在几个波长范围内运行，以确保在几个项目和合作中的广泛适用性。此外，它必须允许长期测量，以研究三维结构中细胞的生长。显微镜系统的一个重要应用是研究由双光子光刻或生物打印方法产生的三维材料性质对细胞、多细胞结构和组织结构的影响。

For characterizing multicellular structures, microscopy techniques are required that ensure high spatial and temporal resolution even several hundred micrometers deep inside a material. This is highly relevant especially in the context of tissue engineering as well as in organotypic 3D tissue culture systems. Furthermore, 3D-structured materials are highly interesting to study cell growth. The microscopic characterization of such biohybrid material cell systems is absolutely necessary to understand the impact of different materials on cell behavior in 3D. Therefore, a multiphoton confocal microscope with high penetration depth and low cytotoxicity is required to carry out projects in which living cellular systems are investigated in three dimensions with high spatial resolution and over longer timescales. The system should operate in several wavelength ranges to ensure broad applicability in several projects and collaborations. In addition, it must allow long-term measurements to study the growth of cells in three-dimensional structures. An important application of the microscopy system is the investigation of the influence of three-dimensional material properties, generated by two-photon lithography or bio-printing methods, on cells, multicellular structures and tissue structures.