Cell and Tissue Microscopy Core

摘要 细胞和组织显微镜核心(CTMC)将支持DC-IDDRC研究人员依赖于 关于使用高分辨率光学和电子显微镜方法来研究细胞和组织。CTMC 将提供最先进的显微成像和分析工具，以支持专注于 儿科疾病，重点是神经发育障碍、胎儿和新生儿脑损伤以及 遗传病。CTMC将向乔治国立儿童医院的研究人员提供服务 华盛顿大学、乔治城大学和霍华德大学。在这些用户中，有一个不断增长的 需要能够以更高的空间和时间分辨率进行成像的工具，同时还允许 成像和深部成像以及大面积成像。为了支持这些需求，CTMC继续扩展其 服务包括宽视场、共焦、带光遗传操作的实时和多光子显微镜、光 薄片显微镜、超分辨率、相关光和EM以及大视场3D-EM。有了这些 核心将继续满足发展，并扩大其使命，以使结构和功能 从纳米级到生物级的成像，旨在了解和治疗导致 IDDS。该核心还将帮助开发和实施图像可视化的量化分析方法 和数据分析。核心还将参与与IDD相关的协作项目，这些项目严重依赖 使用更新的技术。该核心的一项重大使命将是培训下一代研究人员 通过提供动手培训和教育讲习班，使用先进的显微镜方法 由核心与大华盛顿地区的其他公司和研究机构合作组织。CTMC 还将与包括人类和动物成像核心在内的其他DC-IDDRC核心合作， 神经行为核心和基因组学和生物信息学核心，以服务于具有跨学科需求的项目。 通过上述努力，CTMC的目标是继续利用显微镜技术的发展来 使这些发展的概念、设计和实施能够应对IDD领域的挑战 研究和推进DC-IDDRC的使命。

ABSTRACT The Cell and Tissue Microscopy Core (CTMC) will support the DC-IDDRC researchers in their work that relies on the use of high-resolution optical and electron microscopic approaches to study cells and tissues. CTMC will offer access to state-of-the-art microscopy imaging and analytical tools to support research focused on pediatric diseases with an emphasis on neurodevelopmental disorders, fetal and neonatal brain injury and genetic diseases. CTMC will extend its services to researchers at Children’s National Hospital, George Washington University, Georgetown University and Howard University. Among these users, there is a growing need for tools that enable imaging at greater spatial and temporal resolution while also allowing functional imaging and deep as well as large area imaging. To support these needs CTMC continues to expand its services including widefield, confocal, live and multiphoton microscopy with optogenetic manipulation, light sheet microscopy, super resolution, correlative light and EM as well as large field 3D-EM. With these developments the core will continue to meet and also expand its mission to enable structural and functional imaging from nanoscale to organismal scale that are aimed at understanding and treating defects leading to IDDs. The core will also help develop and implement quantitative analytical approaches for image visualization and data analysis. The core will also participate in collaborative IDD-relevant projects that heavily rely on the use of newer technologies. A significant mission of the core will be to train next generation of researchers in the use of advanced microscopy approaches through offering hands on training, educational workshops organized by the core in collaboration with other companies and researches in the greater DC region. CTMC will also collaborate with other DC-IDDRC cores including the Human and Animal Imaging Core, Neurobehavioral Core and Genomics and Bioinformatics Core to serve projects with cross disciplinary needs. Through above endeavors CTMC aims to continue leveraging technological developments in microscopy to enable conception, design, and implementation of these developments to tackle challenges in the field of IDD research and further the mission of DC-IDDRC.