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Circadian regulation of brain and body in larval zebrafish

斑马鱼幼体大脑和身体的昼夜节律调节

基本信息

批准号：
10798875
负责人：
DAVID Edward EHRLICH
金额：
$ 25万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10798875
关键词：
3-Dimensional Ablation Activity Cycles Affect Animal Behavior Animals Arousal Award Behavior Behavioral Biomechanics Brain Brain Stem Calcium Cells Chronobiology Circadian Rhythms Complement Complex Confocal Microscopy Darkness Disease Equipment Fishes Fluorescence Funding Goals Hormones Image Individual Institution Laboratories Lasers Lesion Light Lighting Measurement Measures Metabolism Methods Microscope Microscopy Modeling Muscle Nervous System Neurology Neuromodulator Neurons Neuropeptides Nuclear Optics Organ Output Pacemakers Phase Photons Photosensitizing Agents Physiological Physiology Population Postdoctoral Fellow Research Research Design Resolution Resources Services Signal Pathway Signal Transduction Signaling Molecule Slave Sleep disturbances Specificity Structure System Time Tissues Transgenic Organisms Visible Radiation Wakefulness Work Zebrafish calcium indicator cellular targeting circadian circadian regulation experience experimental study fluorophore genetic approach hypocretin instructor multi-photon multiphoton microscopy muscle physiology neural receptor sleep behavior time use vibration

项目摘要

Project Summary Circadian rhythms drastically alter animal behavior through diverse actions on cellular targets throughout the body. Disruptions of sleep-activity cycles account for a wide range of diseases affecting behavior, neurology, metabolism, and muscle physiology. This proposal presents a set of studies designed to understand the mechanisms and interactions of circadian effects on cells and systems spanning the body. Circadian rhythms manifest downstream of pacemakers via signaling molecules that act directly on targets to regulate physiology. Understanding how distinct targets are modified to achieve a constellation of physiological and behavioral rhythms is a major goal in chronobiological research. We study how neurons, muscles, and biomechanics interact in larval zebrafish, a tractable diurnal vertebrate, and our preliminary experiments suggest these animals experience a breadth of circadian changes far more diverse than previously known. Although the zebrafish is an imperfect model of sleep behavior, its clear circadian rhythms combined with its tractability for physiological, behavioral, and genetic approaches make it an ideal system for understanding how circadian rhythms organize and interact across cells and organs. The original proposal aims to define how diverse circadian effects on nervous system output and muscle physiology amount to complex behavioral output, by tracking and modeling zebrafish behavior and arousal across the diel cycle. Here we request resources to complement institutional support for a multiphoton microscope, which affords activity measurements using invisible light, thereby facilitating comparisons of physiology across circadian time without the confounding effects of measurement approaches that use visible light. Together these experiments will provide detailed information and models regarding the interaction of circadian effects across cells and systems.

项目总结