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Circadian regulation of brain and body in larval zebrafish

斑马鱼幼体大脑和身体的昼夜节律调节

基本信息

批准号：
10798875
负责人：
DAVID Edward EHRLICH
金额：
$ 25万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-15 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10798875
关键词：
3-Dimensional Ablation Activity Cycles Affect Animal Behavior Animals Arousal Award Behavior Behavioral Biomechanics Brain Brain Stem Calcium Cells Chronobiology Circadian Rhythms Complement Complex Confocal Microscopy Darkness Disease Equipment Fishes Fluorescence Funding Goals Hormones Image Individual Institution Laboratories Lasers Lesion Light Lighting Measurement Measures Metabolism Methods Microscope Microscopy Modeling Muscle Nervous System Neurology Neuromodulator Neurons Neuropeptides Nuclear Optics Organ Output Pacemakers Phase Photons Photosensitizing Agents Physiological Physiology Population Postdoctoral Fellow Research Research Design Resolution Resources Services Signal Pathway Signal Transduction Signaling Molecule Slave Sleep disturbances Specificity Structure System Time Tissues Transgenic Organisms Visible Radiation Wakefulness Work Zebrafish calcium indicator cellular targeting circadian circadian regulation experience experimental study fluorophore genetic approach hypocretin instructor multi-photon multiphoton microscopy muscle physiology neural receptor sleep behavior time use vibration

项目摘要

Project Summary Circadian rhythms drastically alter animal behavior through diverse actions on cellular targets throughout the body. Disruptions of sleep-activity cycles account for a wide range of diseases affecting behavior, neurology, metabolism, and muscle physiology. This proposal presents a set of studies designed to understand the mechanisms and interactions of circadian effects on cells and systems spanning the body. Circadian rhythms manifest downstream of pacemakers via signaling molecules that act directly on targets to regulate physiology. Understanding how distinct targets are modified to achieve a constellation of physiological and behavioral rhythms is a major goal in chronobiological research. We study how neurons, muscles, and biomechanics interact in larval zebrafish, a tractable diurnal vertebrate, and our preliminary experiments suggest these animals experience a breadth of circadian changes far more diverse than previously known. Although the zebrafish is an imperfect model of sleep behavior, its clear circadian rhythms combined with its tractability for physiological, behavioral, and genetic approaches make it an ideal system for understanding how circadian rhythms organize and interact across cells and organs. The original proposal aims to define how diverse circadian effects on nervous system output and muscle physiology amount to complex behavioral output, by tracking and modeling zebrafish behavior and arousal across the diel cycle. Here we request resources to complement institutional support for a multiphoton microscope, which affords activity measurements using invisible light, thereby facilitating comparisons of physiology across circadian time without the confounding effects of measurement approaches that use visible light. Together these experiments will provide detailed information and models regarding the interaction of circadian effects across cells and systems.

项目摘要昼夜节律通过对细胞靶标的不同作用极大地改变动物的行为。尸体。睡眠活动周期的中断是影响行为、神经学、新陈代谢和肌肉生理学。该提案提出了一系列研究，旨在了解人体细胞和系统的昼夜节律效应的机制和相互作用。昼夜节律通过直接作用于靶点以调节生理的信号分子，在起搏器下游表现出来。了解如何修改不同的目标以实现一系列生理和行为节律是时间生物学研究的一个主要目标。我们研究神经元、肌肉和生物力学斑马鱼幼体与斑马鱼相互作用，斑马鱼是一种易驯服的日间脊椎动物，我们的初步实验表明，这些动物体验的昼夜节律变化的广度比以前所知的要多样化得多。虽然斑马鱼是一种睡眠行为的不完美模型，它清晰的昼夜节律，以及它对生理的易控性，行为学和遗传学方法使其成为了解昼夜节律如何组织的理想系统并跨越细胞和器官相互作用。最初的提案旨在定义不同的昼夜节律对通过跟踪和建模，神经系统输出和肌肉生理学相当于复杂的行为输出斑马鱼的行为和性欲贯穿整个周期。在此，我们请求提供资源，以补充机构支持多光子显微镜，可使用不可见光测量活动，从而促进生理学跨昼夜时间的比较，而不受测量的混杂影响使用可见光的方法。这些实验将一起提供详细的信息和模型关于跨细胞和系统的昼夜节律效应的相互作用。