DEVELOPMENTAL REGULATION IN DROSOPHILA

果蝇的发育调控

• 批准号: 3297984
• 负责人: FRANK LASKI
• 金额: $ 18.77万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3297984
• 关键词: Drosophilidae; alleles; chromosome deletion; developmental genetics; electron microscopy; gene expression; gene interaction; genetic manipulation; genetic regulation; genetic translation; histochemistry /cytochemistry; histogenesis; leg; light microscopy; microinjections; nucleic acid sequence; ovary; point mutation; precursor mRNA; protein structure function; transcription factor; transposon /insertion element

We have recently found that a remarkable remodeling of the internal structure of the Drosophila ovary takes place at the end of the larval period. This is when the partitioning of the ovary into ovarioles is initiated by the formation of regularly spaced terminal filaments inside the ovary. Drawings of the 3rd instar larval ovary after terminal filament formation and of the anterior tip of an adult ovariole are shown below. Our major goal is to study the development of the ovary during larval stages and to understand how the terminal filaments arise. This work will be divided into three parts: 1. Detailed analysis of the morphogenesis of wild type larval ovaries using light and electron microscopy, enhancer trap markers and mosaic analysis. 2. Molecular and genetic characterization of bric-a-brac and fs(2)eoP.L3, two genes required for the proper formation of terminal filaments. 3. A screen for other genes that are involved in the development of the larval ovary. In addition to being required for terminal filament stack formation, bric-a-brac is also required during leg and antenna development. We have discovered that a bric-a-brac mutant fly has a homeotic transformation of distal tarsal segments to a segment 1 identity, and that this transformation is dependent on the dosage of the bric-a-brac gene. In addition, bric-a-brac is expressed in a discontinuous gradient along the proximal-distal axis of the developing leg. As the second part of this grant, we propose to determine how the bric-a-brac gradient of expression is formed and to identify genes that interact with bric-a-brac during leg development.

我们最近发现，内部的一种显著的重塑 果蝇卵巢的结构发生在幼虫的末端 句号。这是将卵巢分割成卵巢小管的时候 由内部规则间隔的末端细丝的形成引发的 卵巢。3龄幼虫卵巢末梢后示意图 图示成体卵巢前端的细丝形成。 下面。我们的主要目标是研究卵巢在 幼虫阶段，并了解末端细丝是如何出现的。这 工作将分为三个部分： 1.对野生型幼虫卵巢形态发生的详细分析 使用光学和电子显微镜、增强子陷阱标记和镶嵌 分析。 2.brc-a-brac和brc-a-brac的分子遗传学特征 FS(2)eoP.L3，末端正常形成所需的两个基因 细丝。 3.筛选参与发育的其他基因。 幼虫卵巢。 除了端丝堆叠形成所需外， 在支腿和天线的开发过程中，也需要金砖四国。我们有 发现一种brc-a-brac突变果蝇有同源异型转化 1的身份，并且这是 转化依赖于brc-a-brac基因的剂量。在……里面 此外，brc-a-brac表示为沿 发育中腿的近端-远端轴线。作为本文的第二部分 Grant，我们建议确定BRIC-A-BRAC表达梯度是如何 并识别在腿部与brc-a-brac相互作用的基因 发展。