DEVELOPMENTAL REGULATION IN DROSPHILLA

果蝇的发育调控

• 批准号: 2900690
• 负责人: FRANK LASKI
• 金额: $ 19.16万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2900690
• 关键词: DNA binding protein; Drosophilidae; alleles; chromosome deletion; developmental genetics; electron microscopy; gene expression; gene interaction; genetic translation; histochemistry /cytochemistry; histogenesis; leg; light microscopy; microinjections; nucleic acid sequence; oogenesis; protein binding; protein structure function; transcription factor; transposon /insertion element

The gene bric a brac (bab) is a transcriptional regulator that is required for the proper development of the ovary, leg and antenna in Drosophila melanogaster. Our long term goal is to have a molecular understanding of how bab functions, and to use bab as an entryway to the investigation of limb and ovary development. We propose to continue our studies by means of the following specific aims: 1. A functional analysis of bab. We have extensive knowledge of the gene structure, genetics and expression pattern of bab. We have identified a target gene for bab (it binds its own locus) and shown that bab can repress its own production. Our current goal is to understand how bab functions at a molecular level. To this we will investigate the consequence of babII protein binding to a promoter. In addition, we will do structure/function analysis of the babII protein, investigating the function of the BTB domain and psq motif. 2. A screen for genes that interact with bab during leg development. bab provides a unique tool in understanding the process of pattern formation in the leg of Drosophila, with specific interest in the formation of the proximal-distal axis. bab is required to give the three central segments of the tarsus their identity, therefore the gene must be involved in the process of interpreting positional cues along the axis. Understanding how bab interacts with other genes to interpret positional information will give insight into the molecular mechanism of proximal- distal axis formation. Using a genetic approach, we will identify genes that interact with bab. These genes will be studied to determine their role in leg development. 3. A genetic and molecular characterization of the ntf (no terminal filaments) gene. We have recently identified that ntf gene, the second gene identified (in addition to bab) that is required for terminal filament formation. Our long term goal is to understand how developmental processes work during ovary development. We are taking a genetic approach to this goal, identifying genes that are required for ovary development and then analyzing these genes. Analysis of bab has told us a great deal about ovary development, the molecular and genetic characterization of the ntf gene should be just as informative.

基因bric a brac(Bab)是一种转录调节因子，它是 卵巢、腿和触角的正常发育所必需的 黑腹果蝇。我们的长期目标是拥有一种分子 了解Bab的功能，并使用Bab作为进入 四肢和卵巢发育的研究。我们建议继续我们的 通过以下具体目标进行研究： 1.Bab的功能分析。我们对此有广泛的了解 Bab的基因结构、遗传学和表达模式。我们有 确定了Bab的一个靶基因(它结合了自己的基因)，并表明 Bab可以压制自己的生产。我们目前的目标是了解 Bab是如何在分子水平上发挥作用的。为此，我们将调查 Babii蛋白与启动子结合的结果。此外，我们 将对Babii蛋白进行结构/功能分析，调查 BTB结构域和PSQ基序的功能。 2.筛选腿部发育过程中与Bab相互作用的基因。巴布 为理解图案形成过程提供了独特的工具 在果蝇的腿上，特别感兴趣的是 近端-远端轴线。巴布被要求给三个中锋 脚踝的片段与它们的身份不同，因此基因一定是 参与沿轴线解释位置线索的过程。 了解Bab如何与其他基因相互作用来解释位置 信息将使我们深入了解近端- 远端轴线形成。使用遗传学方法，我们将识别基因 与巴布互动的人。这些基因将被研究以确定它们的 在腿部发育中的作用。 3.NTF的遗传和分子特征(无末端 花丝)基因。我们最近发现了NTF基因，第二个 确定(除Bab外)终端所需的基因 形成细丝。我们的长期目标是了解 发育过程在卵巢发育过程中起作用。我们要带着 一种实现这一目标的遗传学方法，识别 卵巢发育，然后分析这些基因。分析Bab Has 告诉了我们很多关于卵巢发育的分子和基因 NTF基因的特征应该同样具有信息量。