SYNTHETIC POLYPEPTIDES VIA IN VITRO PROTEIN BIOSYNTHESIS

通过体外蛋白质生物合成合成多肽

• 批准号: 3302387
• 负责人: Sidney Michael Hecht
• 金额: $ 18.6万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3302387
• 关键词: acylation; aminoacyltransferase; dihydrofolate reductase; enzyme mechanism; enzyme structure; enzyme substrate; peptide chemical synthesis; protein biosynthesis; protein folding; protein structure function; proteins; transfer RNA; transferase; tryptophan synthase

The goal of this study is the use of misacylated transfer RNA's in vitro protein biosynthesizing systems to . define the structure features in the aminoacyl moiety that permit its participation in the peptidyltransferase reaction . probe the physiochemical nature of the peptidyltransferase site . effect the elaboration of structurally modified enzymes containing synthetic amino acids at single, predetermined sites

本研究的目的是在体外使用错酰化转移RNA 蛋白质生物合成系统， . 定义了氨酰基部分的结构特征， 允许 其参与肽基转移酶反应 . 探讨肽基转移酶的理化性质 网站 . 影响结构修饰酶的加工 含有单一的、预定的合成氨基酸 网站