CRYSTALLOGRAPHIC STUDIES OF THE LACTOSE REPRESSOR

乳糖抑制剂的晶体学研究

• 批准号: 3303815
• 负责人: MITCHELL LEWIS
• 金额: $ 21.55万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3303815
• 关键词: DNA; DNA binding protein; X ray crystallography; chemical binding; gene expression; genetic regulation; interferometry; isomorphous substitution; lac operon; lactose; mutant; physical model; protein folding; protein purification; protein structure; synthetic nucleotide

The lactose operon has served as the paradigm to study gene regulation. To understand more completely how the operon is regulated we have undertaken the crystallographic structure determination of (1) the lac repressor, (2) the repressor bound to inducer and (3) the repressor in a ternary complex with synthetic operator fragments of DNA plus an anti-inducer. The three dimensional structure of this protein and the complexes will allow us to address such questions as: (1) How does the repressor recognize specific sites on the DNA? (2) How do the inducers and anti-inducers affect the conformation of the repressor? (3) What role does structure play in the allosteric mechanism and how is it used to regulate gene expression? In addition, the three dimensional structure of the lac repressor will provide a framework for correlating and interpreting an extensive body of genetic and chemical data.

乳糖操纵子已经成为研究基因调控的范例。至 更全面地了解我们所承担的操纵者是如何被监管的 (1)乳胶抑制剂，(2)的晶体结构测定 与诱导物结合的抑制子和(3)三元络合物中的抑制子 用人工合成的DNA操作者片段加上抗诱导剂。三位一体 这种蛋白质和复合体的空间结构将使我们能够 解决以下问题： (1)抑制子如何识别DNA上的特定位置？ (2)诱导剂和抗诱导剂对蛋白质构象有何影响 抑制者？ (3)结构在变构机制中起什么作用？ 它用来调节基因表达？ 此外，紫胶抑制物的三维结构将 提供了一个框架来关联和解释广泛的 遗传和化学数据。