PURCHASE OF AN XRAY GENERATOR AND IMAGE PLATE DETECTOR

购买 X 射线发生器和图像板检测器

• 批准号: 2766826
• 负责人: MITCHELL LEWIS
• 金额: $ 40万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-25 至 2000-01-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2766826
• 关键词: X ray; X ray crystallography; bioimaging /biomedical imaging; biomedical equipment purchase; radiation detector

This proposal requests funds to purchase a Rigaku RU-300 X-ray generator with focusing mirrors, an R-AXIS IV image plate system, and an X-stream low temperature system to improve the existing X-ray diffraction facility at the University of Pennsylvania School of Medicine. The original facility was developed for a single crystallographer and his collaborators. In the past three years, the School of Medicine has hired five new faculty who will all be using X-ray crystallography to address important biological problems in macromolecular structure, function, and design. In addition to hiring new faculty, the University and the School of Medicine have invested over one million dollars to renovate 7000 square feet of laboratory space for structural biology, of which 3000 square feet will be specifically dedicated to X-ray crystallography. This new space will house the existing equipment, as well as the new equipment proposed in this grant. The instrumentation will be jointly managed by the P.I. (Dr. Mitch Lewis) and the three co- P.I.'s (Drs. Greg Van Duyne, Pat Loll, and Hillary Nelson), all of whom have established active crystallography programs. A Ph.D. level scientist will be hired during the summer of 1998 to supervise installation of the equipment in the new space, as well as to perform ongoing equipment maintenance, assist users, and train new users. This scientist will also be available to assist other Medical School scientists that are not X-ray diffraction specialists but who wish to initiate crystallographic projects. In support of this request for shared instrumentation funds, the Department of Biochemistry and Biophysics along with the Department of Pharmacology have agreed to provide funds to support the new X-ray facility manager in the Medical School.

该提案要求资金购买带有聚焦镜的 Rigaku RU-300 X 射线发生器、R-AXIS IV 图像板系统和 X-stream 低温系统，以改进宾夕法尼亚大学医学院现有的 X 射线衍射设施。 最初的设施是为一位晶体学家及其合作者开发的。 在过去的三年里，医学院聘请了五名新教师，他们将使用X射线晶体学来解决大分子结构、功能和设计方面的重要生物学问题。 除了聘请新教师外，大学和医学院还投资了超过 100 万美元，翻新了 7000 平方英尺的结构生物学实验室空间，其中 3000 平方英尺将专门用于 X 射线晶体学。 这个新空间将容纳现有设备以及本次拨款中提议的新设备。该仪器将由 P.I. 共同管理。 （Mitch Lewis 博士）和三位副研究员（Greg Van Duyne、Pat Loll 和 Hillary Nelson 博士），他们都建立了活跃的晶体学项目。 博士学位1998年夏季将聘用一级科学家，负责监督新空间内设备的安装，并进行持续的设备维护、协助用户和培训新用户。 该科学家还将为其他不是 X 射线衍射专家但希望启动晶体学项目的医学院科学家提供帮助。为了支持共享仪器资金的请求，生物化学和生物物理学系以及药理学系已同意提供资金来支持医学院新的 X 射线设施经理。