LASER-ASSISTED FTMS ANALYSIS OF HIGH MASS BIOMOLECULES

高质量生物分子的激光辅助 FTMS 分析

• 批准号: 3303797
• 负责人: Charles Lee Wilkins
• 金额: $ 18.14万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3303797
• 关键词: analytical method; biomedical equipment development; chemical structure; gas chromatography; high performance liquid chromatography; interferometry; lasers; mass spectrometry; molecular weight

Support is sought for a project intended to further extend application of Fourier transform mass spectrometry to the analysis of high mass and non-volatile biomolecules. Of particular interest are applications to high resolution accurate mass analysis of intractable non-volatile species with masses 2000 to 50,000 daltons and above. The research will utilize a unique Nicolet 7.2 Tesla FTMS-2000 dual cell FTMS instrument. A modified dual-cell 3 Tesla FTMS- 1000 instrument equipped with supercritical fluid, high performance liquid chromatography, and gas chromatography interfaces, and a single cell 3 Tesla FTMS- 1000 instrument are also available for the research, if needed. Primary goals of the proposed research include investigation of high resolution matrix-assisted laser desorption mass spectrometry of high mass biomolecules and development of efficacious means of obtaining structural information on biomolecules. Alternatives to collision-induced dissociation (which is not consistently effectual in the energy regime available for FTMS or for molecules with masses above 1500 amu) will be investigated. Specific topics to be investigated include: (1) matrix-assisted ultraviolet and infrared laser desorption; (2) laser desorption/photodissociation methods; (3) use of in situ derivatization methods to incorporate metals to serve as analytical chromophores within analytes of interest; and (4) surface-induced dissociation of laser-desorbed ions. It is expected that this research will result in improved understanding of fundamental processes and constraints governing the analysis of high mass molecular species with masses in the 2000 - 100,000 dalton range. A number of analytically useful methods permitting application of FTMS to biomolecule structure determination are expected to result.

寻求支持旨在进一步扩大应用的项目 傅里叶变换质谱分析高质量和 非挥发性生物分子。 特别感兴趣的是应用程序 难处理的非挥发性物质的高分辨率精确质量分析 质量为 2000 至 50,000 道尔顿及以上的物种。 该研究将 利用独特的 Nicolet 7.2 Tesla FTMS-2000 双池 FTMS 仪器。 改进型双电池 3 Tesla FTMS-1000 仪器，配备 超临界流体、高效液相色谱、气相色谱 色谱接口和单细胞 3 Tesla FTMS-1000 如果需要，还可以使用仪器进行研究。 主要目标 拟议的研究包括高分辨率的调查 高质量基质辅助激光解吸质谱 生物分子和获得结构的有效方法的开发 有关生物分子的信息。 碰撞诱导的替代方案 解离（在能量状态下并不总是有效） 可用于 FTMS 或质量超过 1500 amu 的分子） 调查了。 需要研究的具体主题包括：（1） 基质辅助紫外和红外激光解吸； (2)激光 解吸/光解法； (3)利用原位衍生化 掺入金属作为分析发色团的方法 感兴趣的分析物； (4) 表面诱导解离 激光解吸离子。 预计这项研究将改善 了解基本流程和制约因素 质量在 2000 - 100,000 之间的高质量分子种类的分析 道尔顿范围。 许多分析上有用的方法允许 FTMS在生物分子结构测定中的应用有望 到结果。