LASER-ASSISTED FTMS ANALYSIS OF HIGH MASS BIOMOLECULES

寻求对一个旨在进一步扩大 傅里叶变换质谱法用于高质量和高灵敏度的分析 非挥发性生物分子。 特别令人感兴趣的是应用于 难处理非挥发性物质高分辨率精确质量分析 质量在2000到50，000道尔顿及以上的物种。 这项研究将 使用独特的尼科莱7.2特斯拉FTMS-2000双池FTMS仪器。 一种改进的双池3特斯拉FTMS- 1000仪器，配备有 超临界流体、高效液相色谱和气体 色谱接口和单细胞3特斯拉FTMS- 1000 如果需要的话，还可以使用仪器进行研究。 主要目标 建议的研究包括调查高分辨率 基质辅助激光解吸质谱 生物分子和发展有效的手段，获得结构 生物分子的信息。 碰撞诱导的替代方案 解离（在能量状态下并不总是有效 可用于FTMS或质量高于1500 amu的分子）， 研究了 具体研究内容包括：（1） 基质辅助紫外和红外激光解吸;（2）激光 解吸/光解离方法;（3）使用原位衍生化 掺入金属作为分析发色团的方法 感兴趣的分析物;和（4）表面诱导解离 激光解吸离子 预计这项研究将改善 了解管理的基本过程和限制 分析质量在2000 - 100，000之间的高质量分子种类 道尔顿山脉。 一些分析上有用的方法允许 预计FTMS在生物分子结构测定中的应用 结果。

Support is sought for a project intended to further extend application of Fourier transform mass spectrometry to the analysis of high mass and non-volatile biomolecules. Of particular interest are applications to high resolution accurate mass analysis of intractable non-volatile species with masses 2000 to 50,000 daltons and above. The research will utilize a unique Nicolet 7.2 Tesla FTMS-2000 dual cell FTMS instrument. A modified dual-cell 3 Tesla FTMS- 1000 instrument equipped with supercritical fluid, high performance liquid chromatography, and gas chromatography interfaces, and a single cell 3 Tesla FTMS- 1000 instrument are also available for the research, if needed. Primary goals of the proposed research include investigation of high resolution matrix-assisted laser desorption mass spectrometry of high mass biomolecules and development of efficacious means of obtaining structural information on biomolecules. Alternatives to collision-induced dissociation (which is not consistently effectual in the energy regime available for FTMS or for molecules with masses above 1500 amu) will be investigated. Specific topics to be investigated include: (1) matrix-assisted ultraviolet and infrared laser desorption; (2) laser desorption/photodissociation methods; (3) use of in situ derivatization methods to incorporate metals to serve as analytical chromophores within analytes of interest; and (4) surface-induced dissociation of laser-desorbed ions. It is expected that this research will result in improved understanding of fundamental processes and constraints governing the analysis of high mass molecular species with masses in the 2000 - 100,000 dalton range. A number of analytically useful methods permitting application of FTMS to biomolecule structure determination are expected to result.