HIGH FIELD FT MASS SPECTROMETRY FOR BIOMOLECULES

生物分子高场 FT 质谱

• 批准号: 3278395
• 负责人: Charles Lee Wilkins
• 金额: $ 15.75万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-06-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3278395
• 关键词: evaporation; ionization; ions; laser spectrometry; mass spectrometry; phase change

Continued support is requested for a project which will demonstrate the applicability of Fourier transform mass spectrometry to high resolution accurate mass analysis of ion with masses in the 1,500-20,000 dalton range. This will be accomplished using (1) laser desorption; (2) cesium ion bombardment; (3) fast atom bombardment; (4) thermospray techniques for sample volatilization in conjunction with a dual cell differentially-pumped Fourier transform mass spectrometer and an 8T magnet. It is expected that unprecedented high mass resolution at m/z 20,000 or above will be demonstrated. Furthermore, the relative sensitivity, accuracy, and resolution will be determined in comparison with results to be obtained with the same samples on a high performance VG Instruments ABZ-1HF double focussing mass spectrometer. The applicability of the methodologies developed to bipolymer analyses (e.g., polypeptides and polysugars) will be investigated.

要求继续支持一个项目，该项目将展示 傅里叶变换质谱法在高分辨率上的适用性 质量在1500 - 20000道尔顿的离子的精确质量分析 范围 这将使用（1）激光解吸;（2）铯 离子轰击;（3）快速原子轰击;（4）热喷雾技术 样品挥发与双池差泵联用 傅里叶变换质谱仪和8 T磁体。 预计在 前所未有的高质量分辨率（m/z 20，000或更高）， 演示。 此外，相对灵敏度、准确度和 将通过与获得的结果进行比较来确定分离度 在高性能VG仪器ABZ-1HF双 聚焦质谱仪 方法的适用性 发展到二元聚合物分析（例如，多肽和多糖）将是 研究了

项目成果

Laser desorption/Fourier transform mass spectra of glycoalkaloids and steroid glycosides.

配糖生物碱和类固醇糖苷的激光解吸/傅里叶变换质谱。

• DOI: 10.1002/bms.1200130409
• 发表时间: 1986
• 期刊: Biomedical & environmental mass spectrometry
• 作者: Coates,ML;Wilkins,CL
• 通讯作者: Wilkins,CL

Photodissociation of laser-desorbed ions as a structure determination tool.

• DOI: 10.1021/ac00182a010
• 发表时间: 1989-04
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: L. Nuwaysir;C. Wilkins

Gas chromatography/multiphoton ionization Fourier transform mass spectrometry.

气相色谱/多光子电离傅里叶变换质谱。

• DOI: 10.1021/ac00284a027
• 发表时间: 1985
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Sack,TM;McCrery,DA;Gross,ML
• 通讯作者: Gross,ML

Surface-induced dissociation by Fourier transform mass spectrometry.

通过傅里叶变换质谱法进行表面诱导解离。

• DOI: 10.1021/ac00212a017
• 发表时间: 1990
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Ijames,CF;Wilkins,CL
• 通讯作者: Wilkins,CL

Indium-alkene complex ions as reagents for selective chemical ionization.

铟-烯烃络离子作为选择性化学电离试剂。

• DOI: 10.1021/ac00176a018
• 发表时间: 1989
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Chowdhury,AK;Cooper,JR;Wilkins,CL
• 通讯作者: Wilkins,CL