HIGH FIELD FT MASS SPECTROMETRY FOR BIOMOLECULES

要求继续支持一个项目，该项目将展示 傅里叶变换质谱法在高分辨率上的适用性 质量在1500 - 20000道尔顿的离子的精确质量分析 范围 这将使用（1）激光解吸;（2）铯 离子轰击;（3）快速原子轰击;（4）热喷涂技术， 样品挥发与双池差泵联用 傅里叶变换质谱仪和8 T磁体。 预计在 前所未有的高质量分辨率（m/z 20，000或更高）， 演示。 此外，相对灵敏度、准确度和 将通过与获得的结果进行比较来确定分离度 在高性能VG仪器ABZ-1HF双 聚焦质谱仪 方法的适用性 发展到二元聚合物分析（例如，多肽和多糖）将是 研究了

Continued support is requested for a project which will demonstrate the applicability of Fourier transform mass spectrometry to high resolution accurate mass analysis of ion with masses in the 1,500-20,000 dalton range. This will be accomplished using (1) laser desorption; (2) cesium ion bombardment; (3) fast atom bombardment; (4) thermospray techniques for sample volatilization in conjunction with a dual cell differentially-pumped Fourier transform mass spectrometer and an 8T magnet. It is expected that unprecedented high mass resolution at m/z 20,000 or above will be demonstrated. Furthermore, the relative sensitivity, accuracy, and resolution will be determined in comparison with results to be obtained with the same samples on a high performance VG Instruments ABZ-1HF double focussing mass spectrometer. The applicability of the methodologies developed to bipolymer analyses (e.g., polypeptides and polysugars) will be investigated.