LASER-ASSISTED FTMS ANALYSIS OF HIGH MASS BIOMOLECULES

寻求支持一个旨在进一步扩大应用的项目 傅里叶变换质谱学在高质量和高纯度分析中的应用 非挥发性生物分子。特别令人感兴趣的是应用于 高分辨率非挥发性难挥发分的高精度质量分析 质量在2000至50000道尔顿及以上的品种。这项研究将 使用独特的Nicolet 7.2特斯拉FTMS-2000双电池FTMS仪器。 改装的双电池3Tesla FTMS-1000型仪器 超临界流体、高效液相色谱和气体 层析界面，单细胞3特斯拉FTMS-1000 如果需要，也可以使用仪器进行研究。主要目标 建议的研究包括高分辨率的调查 基质辅助高质量激光解吸质谱 生物分子与获得结构的有效手段的发展 关于生物分子的信息。碰撞引发的替代方案 解离(这在能量机制中并不总是有效的 可用于FTMS或质量大于1500 Amu的分子)将是 调查过了。拟考察的具体课题包括：(一) 基质辅助的紫外和红外激光解吸；(2)激光 解吸/光解离方法；(3)原位衍生化的使用 将金属加入作为分析生色团的方法 感兴趣的分析物；以及(4)表面诱导的解离 激光解吸离子。 预计这项研究将导致改进 对基本流程和制约因素的理解 2000-10万年间高质量分子物种的分析 道尔顿山脉。许多分析上有用的方法允许 傅立叶变换质谱仪在生物分子结构测定中的应用前景 才会有结果。

Support is sought for a project intended to further extend application of Fourier transform mass spectrometry to the analysis of high mass and non-volatile biomolecules. Of particular interest are applications to high resolution accurate mass analysis of intractable non-volatile species with masses 2000 to 50,000 daltons and above. The research will utilize a unique Nicolet 7.2 Tesla FTMS-2000 dual cell FTMS instrument. A modified dual-cell 3 Tesla FTMS- 1000 instrument equipped with supercritical fluid, high performance liquid chromatography, and gas chromatography interfaces, and a single cell 3 Tesla FTMS- 1000 instrument are also available for the research, if needed. Primary goals of the proposed research include investigation of high resolution matrix-assisted laser desorption mass spectrometry of high mass biomolecules and development of efficacious means of obtaining structural information on biomolecules. Alternatives to collision-induced dissociation (which is not consistently effectual in the energy regime available for FTMS or for molecules with masses above 1500 amu) will be investigated. Specific topics to be investigated include: (1) matrix-assisted ultraviolet and infrared laser desorption; (2) laser desorption/photodissociation methods; (3) use of in situ derivatization methods to incorporate metals to serve as analytical chromophores within analytes of interest; and (4) surface-induced dissociation of laser-desorbed ions. It is expected that this research will result in improved understanding of fundamental processes and constraints governing the analysis of high mass molecular species with masses in the 2000 - 100,000 dalton range. A number of analytically useful methods permitting application of FTMS to biomolecule structure determination are expected to result.