LASER-ASSISTED FTMS ANALYSIS OF HIGH MASS BIOMOLECULES
LASER-ASSISTED FTMS ANALYSIS OF HIGH MASS BIOMOLECULES
批准号:
3303801
负责人:
Charles Lee Wilkins
金额:
$16.55万
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-05-01 至 1994-04-30
中文摘要
寻求支持一个旨在进一步扩大应用的项目
英文摘要
Support is sought for a project intended to further extend application of
Fourier transform mass spectrometry to the analysis of high mass and
non-volatile biomolecules. Of particular interest are applications to
high resolution accurate mass analysis of intractable non-volatile
species with masses 2000 to 50,000 daltons and above. The research will
utilize a unique Nicolet 7.2 Tesla FTMS-2000 dual cell FTMS instrument.
A modified dual-cell 3 Tesla FTMS- 1000 instrument equipped with
supercritical fluid, high performance liquid chromatography, and gas
chromatography interfaces, and a single cell 3 Tesla FTMS- 1000
instrument are also available for the research, if needed. Primary goals
of the proposed research include investigation of high resolution
matrix-assisted laser desorption mass spectrometry of high mass
biomolecules and development of efficacious means of obtaining structural
information on biomolecules. Alternatives to collision-induced
dissociation (which is not consistently effectual in the energy regime
available for FTMS or for molecules with masses above 1500 amu) will be
investigated. Specific topics to be investigated include: (1)
matrix-assisted ultraviolet and infrared laser desorption; (2) laser
desorption/photodissociation methods; (3) use of in situ derivatization
methods to incorporate metals to serve as analytical chromophores within
analytes of interest; and (4) surface-induced dissociation of
laser-desorbed ions.
It is expected that this research will result in improved
understanding of fundamental processes and constraints governing the
analysis of high mass molecular species with masses in the 2000 - 100,000
dalton range. A number of analytically useful methods permitting
application of FTMS to biomolecule structure determination are expected
to result.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
LASER-ASSISTED FTMS ANALYSIS OF HIGH MASS BIOMOLECULES
-
批准号:3303800
-
项目类别:
-
资助金额:$15.88万
-
财政年份:1991
-
负责人:Charles Lee Wilkins
-
依托单位:
LASER-ASSISTED FTMS ANALYSIS OF HIGH MASS BIOMOLECULES
-
批准号:3303797
-
项目类别:
-
资助金额:$18.14万
-
财政年份:1991
-
负责人:Charles Lee Wilkins
-
依托单位:
LD FTMS OF HIGH MASS BIOMOLECULES
-
批准号:6027513
-
项目类别:
-
资助金额:$6.77万
-
财政年份:1991
-
负责人:Charles Lee Wilkins
-
依托单位:
LD-FTMS OF HIGH MASS BIOMOLECULES
-
批准号:2182609
-
项目类别:
-
资助金额:$18.79万
-
财政年份:1991
-
负责人:Charles Lee Wilkins
-
依托单位:
LD FTMS OF HIGH MASS BIOMOLECULES
-
批准号:2743762
-
项目类别:
-
资助金额:$4.28万
-
财政年份:1991
-
负责人:Charles Lee Wilkins
-
依托单位:
LD-FTMS OF HIGH MASS BIOMOLECULES
-
批准号:2022388
-
项目类别:
-
资助金额:$19.41万
-
财政年份:1991
-
负责人:Charles Lee Wilkins
-
依托单位:
LD-FTMS OF HIGH MASS BIOMOLECULES
-
批准号:2182608
-
项目类别:
-
资助金额:$25.19万
-
财政年份:1991
-
负责人:Charles Lee Wilkins
-
依托单位:
LD-FTMS OF HIGH MASS BIOMOLECULES
-
批准号:2608917
-
项目类别:
-
资助金额:$15.64万
-
财政年份:1991
-
负责人:Charles Lee Wilkins
-
依托单位:
MASS SPECTROMETER/HPLC SYSTEM
-
批准号:3519805
-
项目类别:
-
资助金额:$23.6万
-
财政年份:1987
-
负责人:Charles Lee Wilkins
-
依托单位:
HIGH FIELD FT MASS SPECTROMETRY FOR BIOMOLECULES
-
批准号:3278395
-
项目类别:
-
资助金额:$15.75万
-
财政年份:1982
-
负责人:Charles Lee Wilkins
-
依托单位:
HIGH FIELD FT MASS SPECTROMETRY FOR BIOMOLECULES
-
批准号:3278394
-
项目类别:
-
资助金额:$12.48万
-
财政年份:1982
-
负责人:Charles Lee Wilkins
-
依托单位:
HIGH FIELD FT MASS SPECTROMETRY FOR BIOMOLECULES
-
批准号:3278389
-
项目类别:
-
资助金额:$33.42万
-
财政年份:1982
-
负责人:Charles Lee Wilkins
-
依托单位:
海外基金