GONADAL AND GONADOTROPIN REGULATION IN THE FETUS

胎儿性腺和促性腺激素的调节

基本信息

批准号：
3315872
负责人：
ROBERT B JAFFE
金额：
$ 15.61万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
1987
资助国家：
美国
起止时间：
1987-01-01 至 1989-12-31
项目状态：
已结题

项目摘要

The long-term goal is to gain greater understanding of the role of the human and subhuman primate fetal gonad in sexual development. As an intermediate step leading to this goal, a series of in vitro and in vivo studies are planned. In vitro, it is proposed to study: 1) factors influencing growth of the fetal gonad by assessing the effects of epidermal growth factor, fibroblast growth factor, serum and placental extract on gonadal cell proliferation; 2) binding of gonadotropins to the fetal gonads and correlation of this binding with functional activity of the gonads; 3) facets of hCG formation by the human fetus, including synthesis by the fetal gonad, binding to fetal thyroid membranes and separation of fetal from placental hCG; and 4) possible factors responsible for the apparent lack of desensitization of fetal leydig cells by hCG. In vivo, in the long-term catheterized rhesus monkey fetus, it is proposed to determine: 1) whether there is pulsatile discharge of LH and FSH; 2) the effects of pulsatile administration of gonadotropin-releasing hormone on gonadotropin and gonadal steroid production; and 3) the possible modulating effects of opiate peptides and other modulators on gonadotropin production. As gonadal function, at least of the testis, is necessary for normal sexual development, and as gonadotropin-gonadal interactions differ significantly between primates and other experimental animals, greater understanding of these phenomena in the primate has significant biomedical relevance.

长期目标是对人类和亚人类灵长类动物性胎儿性腺在性发展中。作为导致这一目标的中间步骤，一系列体外和体内计划进行研究。在体外，建议研究：1）因素通过评估表皮的影响来影响胎儿性腺的生长生长因子，成纤维细胞生长因子，血清和胎盘提取物性腺细胞增殖； 2）促性腺激素与胎儿性腺结合这种结合与性腺功能活性的相关性； 3）人类胎儿的HCG形成方面，包括由胎儿性腺，与胎儿甲状腺膜结合和胎儿分离来自胎盘HCG； 4）可能导致明显的因素 HCG缺乏对胎儿Leydig细胞的脱敏。在体内，在长期导管鼠尾草胎儿，建议确定： 1）LH和FSH是否有脉冲排放； 2）促性腺激素释放激素对促性腺激素释放激素的搏动性给药和性腺类固醇产生； 3）阿片类肽和其他调节剂的促性腺激素产生。由于性腺功能至少是睾丸的功能，对于正常性是必要的发展，并且随着促性腺激素 - 基达尔的相互作用显着差异在灵长类动物和其他实验动物之间，对灵长类动物中的这些现象具有显着的生物医学相关性。