HCG IN NORMAL HUMANS

正常人的 HCG

• 批准号: 3316138
• 负责人: WILLIAM D ODELL
• 金额: $ 9.71万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3316138
• 关键词: adrenocorticotropic hormone; blood chemistry; cell type; chorionic gonadotropin; diagnosis design /evaluation; endocrine disorder diagnosis; female reproductive system disorder diagnosis; follicle stimulating hormone; gonadotropin releasing factor; human subject; human tissue; immunologic assay /test; laboratory mouse; laboratory rabbit; luteinizing hormone; monoclonal antibody; postmenopause; prolactin; protein sequence; radioimmunoassay; radiotracer; thyrotropin; urinalysis

We have developed very sensitive and specific, two-monoclonal, antibody sandwich type assays for human thyrotropin, human luteinizing hormone (hLH), human chorionic gonadotropin (hCG), and human prolactin. Using the assays for hCG and hLH we have quantified hCG in unextracted blood of all normal non pregnant women, post-menopausal women, and men. Our data suggest hCG is secreted by the pituitary gland. and that hCG is secreted in pulsatile fashion in parallel with hLH in women. We now propose continued research in two broad areas: (A) continued technical assay development - for ACTH, human follicle stimulating hormone (hFSH), and human alpha chain, and (B) characterization of the "hCG-like" material secreted in normals. This includes the following: (1) characterization of the pulsatile dynamics and control of hCG in normal humans and humans with disorders of the hypothalamic-pituitary-gonadal system; (2) determination of the pituitary cell-type secreting hCG in normals; (3) physico-chemical characterization and determination of the amino acid sequence of hCG purified from pituitary, plasma and urine of normals; (4) physico-chemical characterization and determination of amino acid sequence of the "hCG-like material produced by bacteria with comparison to that produced by normal humans.

我们已经开发了非常敏感和特异的，双单克隆， 人促甲状腺素抗体夹心型测定法 促性腺激素（hLH）、人绒毛膜促性腺激素（hCG）和 人催乳素 使用hCG和hLH的测定，我们得到 在所有正常非妊娠的未提取的血液中定量hCG 女性、绝经后女性和男性。 我们的数据表明hCG是 由脑下垂体分泌而hCG是由 与女性hLH的搏动方式平行。 我们现建议 继续在两个广泛领域进行研究：（A）继续技术研究 测定开发-用于ACTH、人促卵泡激素 （B）人促卵泡激素（hFSH）和人α链的特征，和（B）人促卵泡激素（hFSH）和人α链的特征。 正常人分泌的“hCG样”物质。 这包括 以下内容：（1）脉动动力学的表征， 在正常人和患有疾病的人中的hCG控制 下丘脑-垂体-性腺系统;（2）测定 正常人垂体分泌hCG细胞型 的氨基酸序列的表征和测定 从正常人垂体、血浆和尿液中纯化hCG; 氨基酸的理化表征和测定 “由细菌产生的hCG样物质”的序列， 与正常人产生的相比。