EXTREMELY RAPID GONADOTROPIN ASSAYS

极快速促性腺激素测定

• 批准号: 3316137
• 负责人: WILLIAM D ODELL
• 金额: $ 8.39万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3316137
• 关键词: antibody; chorionic gonadotropin; endocrine disorder diagnosis; female reproductive system disorder diagnosis; fertility; follicle stimulating hormone; immunologic assay /test; luteinizing hormone; male reproductive system disorder diagnosis; monoclonal antibody; radioimmunoassay; radiotracer; rapid diagnosis

Problems of both population control, and diagnosis and treatment of infertility, are of major importance to today's society. Integral to caring for such matters are assay systems for pituitary and gonadal hormones that control reproductive functions. Currently, such assays usually require 2 to 4 days to perform. Recently, by optimizing conventional competitive binding immunoassay techniques for human luteinizing hormone (hLH), we have developed an assay that requires only 2 hours to perform. This assay is currently in routine use in our hospital. We now propose to develop so-called "two-site" noncompetitive assays for human LH, follicle stimulating hormone, and chorionic gonadotropins that can be performed in 15 minutes. Such assays would utilize highly selected monoclonal antibodies labeled with 125I (or later enzyme labeled) as a first reagent. This reagent is used in "great excess," permitting rapid reaction. A second polyclonal antibody directed against multiple other determinants on the hormone is used to separate labeled "bound" monoclonal antibody from "free." Such "short turn-around" assays will permit evaluation of a patient during the initial clinic visit, as well as determination of the ovulatory surge during its occurrence. Such methods would be widely applicable and usable in Obstetrical/Gynecological centers and Endocrine centers treating infertility, and for the control of fertility.

人口控制和疾病诊治问题 不孕不育症对当今社会具有重大意义。 积分到 处理这些问题的是垂体和性腺的检测系统 控制生殖功能的激素。 目前，此类检测 通常需要2到4天才能执行。 最近，通过优化 人类常规竞争性结合免疫测定技术 黄体生成素 (hLH)，我们开发了一种检测方法，只需 2 执行时间。 该检测目前在我们医院常规使用。 我们现在建议开发所谓的“双位点”非竞争性检测方法 人类 LH、卵泡刺激素和绒毛膜促性腺激素 15分钟内即可完成。 此类测定将利用高度精选的 用 125I 标记（或后来的酶标记）的单克隆抗体作为 第一试剂。 该试剂“大量过量”使用，可快速 反应。 针对多种其他抗体的第二种多克隆抗体 激素上的决定簇用于分离标记的“结合”单克隆抗体 抗体来自“游离”。 这种“短周转”测定将允许 在初次就诊期间对患者的评估，以及 确定排卵高峰发生期间的情况。 此类方法 将广泛适用于妇产科中心 和内分泌中心治疗不孕症，并控制 生育能力。