HCG IN NORMAL HUMANS

正常人的 HCG

• 批准号: 3316132
• 负责人: WILLIAM D ODELL
• 金额: $ 10.33万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3316132
• 关键词: adrenocorticotropic hormone; blood chemistry; cell type; chorionic gonadotropin; diagnosis design /evaluation; endocrine disorder diagnosis; female reproductive system disorder diagnosis; follicle stimulating hormone; gonadotropin releasing factor; human subject; human tissue; immunologic assay /test; laboratory mouse; laboratory rabbit; luteinizing hormone; monoclonal antibody; postmenopause; prolactin; protein sequence; radioimmunoassay; radiotracer; thyrotropin; urinalysis

We have developed very sensitive and specific, two-monoclonal, antibody sandwich type assays for human thyrotropin, human luteinizing hormone (hLH), human chorionic gonadotropin (hCG), and human prolactin. Using the assays for hCG and hLH we have quantified hCG in unextracted blood of all normal non pregnant women, post-menopausal women, and men. Our data suggest hCG is secreted by the pituitary gland. and that hCG is secreted in pulsatile fashion in parallel with hLH in women. We now propose continued research in two broad areas: (A) continued technical assay development - for ACTH, human follicle stimulating hormone (hFSH), and human alpha chain, and (B) characterization of the "hCG-like" material secreted in normals. This includes the following: (1) characterization of the pulsatile dynamics and control of hCG in normal humans and humans with disorders of the hypothalamic-pituitary-gonadal system; (2) determination of the pituitary cell-type secreting hCG in normals; (3) physico-chemical characterization and determination of the amino acid sequence of hCG purified from pituitary, plasma and urine of normals; (4) physico-chemical characterization and determination of amino acid sequence of the "hCG-like material produced by bacteria with comparison to that produced by normal humans.

我们已经开发出非常敏感和特异的双单克隆，