PROPERTIES OF UTERINE EPITHELIUM RELATED TO IMPLANTATION

这项研究将检查子宫上皮细胞的特性， 质膜，其与细胞调节的 胚泡粘附和着床的接受条件。 孕酮和雌二醇诱导的上皮细胞膜改变 性质（表面电荷，特异性凝集素结合，糖萼结构， 糖蛋白/多肽谱）。 针对阶段特异性膜蛋白的单特异性抗血清将是 产生并用作上皮细胞接受状态的探针， 在体内和体外模型系统中。 漂浮胶原蛋白凝胶用于 子宫上皮细胞的培养允许保留细胞的 在体内观察表型。 类固醇激素和 子宫内膜间质细胞对上皮细胞分化的影响 感受性的膜标记物的出现将在本研究中进行研究。 培养体系 膜组分在细胞膜中的功能作用 与滋养层的相互作用将使用细胞粘附试验来探索， “接受性”和“非接受性”上皮培养物。 这项研究将 测试子宫上皮是子宫内膜的屏障这一假设， 非接受性子宫中的滋养层附着， 上皮细胞质膜中的上皮细胞介导的变化对于 从而允许在卵细胞植入时粘附和附着。 明确 子宫和胚胎之间同步发展的机制是 对理解植入过程至关重要，因此， 人类和其他动物的生育调节。

The research proposed will examine properties of the uterine epithelial plasma membrane which correlate with the hormone-regulated acquisition of the receptive condition to blastocyst adhesion and implantation. Progesterone- and estradiol-induced alterations in epithelial membrane properties (suface charge, specific lectin binding, glycocalyx structure, glycoprotein/polypeptide profile) have been defined for the rabbit uterus. Monospecific antisera against stage-specific membrane proteins will be produced and used as probes for the receptive state of the epithelium both in vivo and in an in vitro model system. Use of floating collagen gels for culture of uterine epithelial cells allows for retention of the cellular phenotype observed in vivo. The effects of steroid hormones and of endometrial stromal cells on the differentiation of epithelium and on the appearance of membrane markers of receptivity will be studied in this culture system. The functional role of membrane components in the interaction with trophoblast will be explored using cell adhesion assays on "receptive" and "non-receptive" epithelial cultures. This research will test the hypothesis that the uterine epithelium is the barrier to trophoblast attachment in the non-receptive uterus and that hormone-mediated changes in the epithelial plasmalemma are crucial to allowing adhesion and attachment at ovo-implantation. Clarifying the mechanisms for development of synchrony between the uterus and embryo is vital to understanding the implantation process and, as such, has relevance to fertility regulation in both humans and other animal species.