CORE--MORPHOLOGY AND MOLECULAR BIOLOGY

核心--形态学和分子生物学

• 批准号: 6440551
• 负责人: LOREN H HOFFMAN
• 金额: $ 17.42万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440551
• 关键词: bioimaging /biomedical imaging; biomedical facility; complementary DNA; computer assisted sequence analysis; cooperative study; cryopreservation; digital imaging; electron microscopy; expression cloning; fluorescence microscopy; genetic library; histology; immunocytochemistry; immunofluorescence technique; light microscopy; molecular biology; nucleic acid probes; photomicrography; scanning electron microscopy; transfection; transmission electron microscopy; uterus

Description (Adapted from Applicant's Description): The objective of the Morphology Core will be to provide access to a variety of electron and light microscopic (EM and LM, respectively) services and equipment. Specialized cytochemical protocols worked our for reproductive biology tissues will be applied to support key center projects. In addition, there will be routine transmission and scanning electron microscopy and light microscopy, including cryosectioning, brightfield, phase contrast, DIC and immunofluorescence. The Core will also assist Center investigators in the design of experimental protocols that involve cytochemistry or morphology. The Core will also prepare images for publication. Finally, the Core aims include some developmental work, making the new methods available to Center investigators.

描述(改编自申请者的描述)： 形态的核心将是提供对各种电子和 光学显微镜(分别为EM和LM)服务和设备。 专门的细胞化学方案对生殖生物学起作用 组织将被应用于支持重点中心项目。此外, 将会有常规的透射和扫描电子显微镜和 光学显微镜，包括冰冻切片，明场，位相对比， DIC和免疫荧光。核心还将协助中心 参与实验方案设计的研究人员涉及 细胞化学或形态。核心还将为以下项目准备图像 出版。最后，核心目标包括一些开发工作， 将新方法提供给中心的调查人员。