PROPERTIES OF UTERINE EPITHELIUM RELATED TO IMPLANTATION

拟议研究的目标是确定和描述 子宫上皮表面的性质，涉及到 植入过程中滋养层细胞的黏附和随后的侵袭。这个 研究将检验上皮起主要作用的假设 调节子宫受激素调节的接受性的部位 胚泡着床。该项目的目标包括1)使用 生化方法鉴定和分离子宫和滋养层细胞 在互补中特定地相互结合的膜分子 受体/配体相互作用，2)验证结构和生化 体外子宫上皮系统作为子宫内膜癌模型的特性 可接受的子宫表面，并适当改进系统，以及3) 用上皮细胞培养法检测滋养层-子宫粘连 为了测试识别的受体/配体分子的功能作用 根据目标1.到目前为止所研究的模式提供了允许 细胞-细胞所需母体和胚胎因子的鉴定 这些遗传上不同的组织之间的粘连和融合。结果 将适用于阐明细胞相互作用中的一般问题 更具体地说，是关于确定怀孕的过程。

The objective of the proposed research is to identify and characterize properties of the epithelial surface of the uterus which are involved in trophoblast adhesion and subsequent invasion during implantation. The research will test the hypothesis that the epithelium serves as the primary site for mediation of hormonally-modulated receptiveness of the uterus to blastocyst implantation. Aims of the project include 1) the use of biochemical methods to identify and isolate uterine and trophoblast membrane molecules which bind specifically to one another in complementary receptor/ligand interactions, 2) to validate the structural and biochemical properties of an in vitro uterine epithelial system as a model for the receptive uterine surface, and to refine the system as appropriate, and 3) to use the epithelial cultures to examine trophoblast-uterine adhesion and to test the functional roles of the receptor/ligand molecules identified under Aim 1. The model, as studied to date, offers promise for allowing identification of maternal and embryonic factors required for cell-cell adhesion and fusion between these genetically disparate tissues. Results will be applicable to elucidating problems in cell-interaction generally and, more specifically, to the process of establishing pregnancy.