CORE--MORPHOLOGY AND MOLECULAR BIOLOGY

核心--形态学和分子生物学

• 批准号: 6346205
• 负责人: LOREN H HOFFMAN
• 金额: $ 17.29万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6346205
• 关键词: bioimaging /biomedical imaging; biomedical facility; complementary DNA; computer assisted sequence analysis; cooperative study; cryopreservation; digital imaging; electron microscopy; expression cloning; fluorescence microscopy; genetic library; histology; immunocytochemistry; immunofluorescence technique; light microscopy; molecular biology; nucleic acid probes; photomicrography; scanning electron microscopy; transfection; transmission electron microscopy; uterus

Description (Adapted from Applicant's Description): The objective of the Morphology Core will be to provide access to a variety of electron and light microscopic (EM and LM, respectively) services and equipment. Specialized cytochemical protocols worked our for reproductive biology tissues will be applied to support key center projects. In addition, there will be routine transmission and scanning electron microscopy and light microscopy, including cryosectioning, brightfield, phase contrast, DIC and immunofluorescence. The Core will also assist Center investigators in the design of experimental protocols that involve cytochemistry or morphology. The Core will also prepare images for publication. Finally, the Core aims include some developmental work, making the new methods available to Center investigators.

描述（改编自申请人的描述）： Morphology Core将提供各种电子和 光学显微镜（分别为EM和LM）服务和设备。 专门的细胞化学方案为生殖生物学工作 组织用于支持中心重点项目。此外，本发明还提供了一种方法， 将有常规的透射和扫描电子显微镜， 光学显微镜，包括冷冻切片，明场，相差， DIC和免疫荧光。核心还将协助中心 研究人员在设计实验方案，涉及 细胞化学或形态学。核心还将准备图像， 出版物最后，核心目标包括一些发展工作， 使中心的研究人员能够使用新的方法。