NUCLEO-CYTOPLASMIC INTERACTIONS IN DEVELOPMENT

发育中的核质相互作用

• 批准号: 3314736
• 负责人: DAVOR SOLTER
• 金额: $ 13.87万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1986-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3314736
• 关键词: alleles; cell cell interaction; cell fusion; cell nucleus; cytogenetics; cytoplasm; developmental genetics; early embryonic stage; embryo /fetus culture; gene expression; genotype; gestational age; glucose 6 phosphate isomerase; immunofluorescence technique; lethal genes; mammalian embryology; oogenesis; phosphoglycerate kinase; sex chromosomes; sex linked trait; surface antigens

The respective roles of nucleus and cityoplasm in early mammalian development are little understood. What effect maternally inherited cytoplasmic components have on differentiation, and the developmental stage to which they persist are largely unknown. We propose to investigate nuclear/cytoplasmic interactions in the mouse embryo using a novel nuclear tranplantation technique. This technique will be employed to determine the ability of nuclear transplant empryos to undergo normal development and also define the developmental stage at which nuclear endoced gene products can first be detected. Possible maternally derived cytoplasmic contributions to the embryonic phenotype will also be investigated. Experiments regarding the latter were specifically chosen to define possible cytoplasmic effects at different stages of development (ranging from early preimplantation development to late gestational embryogenesis). It is hoped that these experiments will delineate the nuclear/cytoplasmic origin of embryonic gene products and events in addition to increasing our understanding of the biology of nuclear transplantation in mammals.

细胞核和胞质在早期哺乳动物中的作用 发展很少被了解。 什么影响母系遗传 细胞质成分的分化，发育阶段 他们所坚持的基本上是未知的。 我们建议调查 用一种新的核内蛋白质在小鼠胚胎中的核/胞质相互作用 移植技术 该技术将用于确定 核移植胚胎进行正常发育的能力， 还定义了核内基因产物的发育阶段， 可以首先检测到。 可能为母源性细胞质 还将研究对胚胎表型的贡献。 关于后者的实验被特别选择来定义 可能的细胞质效应在不同的发展阶段（范围 从早期植入前发育到晚期妊娠胚胎发生）。 希望这些实验将描绘细胞核/细胞质 胚胎基因产物和事件的起源，除了增加我们的 了解哺乳动物核移植的生物学。