NUCLEO-CYTOPLASMIC INTERACTIONS IN DEVELOPMENT

细胞核和胞浆在早期哺乳动物中的各自作用 人们对发展知之甚少。母性遗传的影响是什么 细胞质成分有分化、分化和发育阶段 他们坚持的目标在很大程度上是未知的。我们建议调查 利用一种新的核在小鼠胚胎中的核/胞质相互作用 移植技术。这项技术将被用于确定 核移植胚胎的正常发育和能力 也定义了核内化基因产物的发育阶段 可以首先被检测到。可能是母源细胞质 对胚胎表型的贡献也将被调查。 关于后者的实验是专门选择的，以确定 不同发育阶段可能的细胞质效应(Range 从早期植入前发育到晚期妊娠胚胎发育)。 人们希望这些实验将勾勒出核质 胚胎基因产品和事件的起源除了增加我们的 对哺乳动物核移植生物学的了解。

The respective roles of nucleus and cityoplasm in early mammalian development are little understood. What effect maternally inherited cytoplasmic components have on differentiation, and the developmental stage to which they persist are largely unknown. We propose to investigate nuclear/cytoplasmic interactions in the mouse embryo using a novel nuclear tranplantation technique. This technique will be employed to determine the ability of nuclear transplant empryos to undergo normal development and also define the developmental stage at which nuclear endoced gene products can first be detected. Possible maternally derived cytoplasmic contributions to the embryonic phenotype will also be investigated. Experiments regarding the latter were specifically chosen to define possible cytoplasmic effects at different stages of development (ranging from early preimplantation development to late gestational embryogenesis). It is hoped that these experiments will delineate the nuclear/cytoplasmic origin of embryonic gene products and events in addition to increasing our understanding of the biology of nuclear transplantation in mammals.