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SYNTHESIS AND SECRETION OF GNRH BY THE FETAL PLACENTA

胎儿胎盘合成和分泌 GNRH

基本信息

批准号：
3317356
负责人：
JANICE M BAHR
金额：
$ 5.04万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-12-01 至 1986-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3317356
关键词：
cell type chorionic gonadotropin corpus luteum gestational age gonadotropin releasing factor high performance liquid chromatography hormone biosynthesis immunofluorescence technique immunoprecipitation molecular sieving organ culture placenta pregnancy radioimmunoassay statistics /biometry

项目摘要

In the pregnant rabbit doe, follicular estrogens and the fetal placenta are required to maintain progesterone production by the corpora lutea (CL) during gestation. The fetal placenta exerts its effects on the CL through either an antiluteolytic and/or luteotropic mechanism. However, studies examining the gonadotropin-like activity of rabbit placentas have failed to identify any chorionic gonadotropin. Recent evidence suggests that the placenta may act as an indirect luteotropin by modifying pituitary or follicular secretions. We have identified a small peptide with both biological and immunological GnRH-like activity in the rabbit placenta. It is not clear whether the placenta synthesizes this molecule or merely binds GnRH found in the peripheral circulation. The specific aims of this proposal are: 1) to determine whether the rabbit fetal placenta synthesizes GnRH; 2) to determine whether GnRH is secreted by the rabbit placenta; 3) to localize the site of synthesis or binding of GnRH within the placenta; and 4) to quantitate changes in the levels of GnRH in the placenta throughout pregnancy. Synthesis of GnRH will be determined by incubating placentas with 3H-leucine and measuring the formation of 3H-GnRH in culture medium. Secretion of GnRH will be determined using a perifusion system and measuring the release of GnRH by placental tissue in response to pulses of 60mM K+. Localization of GnRH within the placenta will be achieved through immunofluorescence using a GnRH antibody linked to a fluorescent compound. Finally, placentas collected at days 12, 18, and 28 of gestation will be analyzed for GnRH content. These studies will extend our understanding of mechanisms involved in the maintenance of pregnancy. In addition, they will provide new information concerning extrahypothalamic sources of GnRH.

在怀孕的母兔中，卵泡雌激素和胎儿胎盘维持黄体 (CL) 产生黄体酮所需妊娠期间。胎儿胎盘通过以下方式对 CL 产生影响：抗黄体溶解和/或黄体促黄体机制。然而，研究检查兔胎盘的促性腺激素样活性未能识别任何绒毛膜促性腺激素。最近的证据表明胎盘可以通过改变垂体或间接发挥促黄体素的作用。卵泡分泌物。我们已经鉴定出一种小肽兔胎盘中 GnRH 样生物和免疫活性。它尚不清楚胎盘是否合成该分子或仅结合 GnRH 存在于外周循环中。本次活动的具体目标建议是： 1）判断胎盘是否为兔胎儿合成GnRH； 2）确定GnRH是否是兔子分泌的胎盘; 3) 定位GnRH的合成或结合位点胎盘； 4) 量化 GnRH 水平的变化整个怀孕期间的胎盘。 GnRH 的合成将由下式确定将胎盘与 3H-亮氨酸一起孵育并测量 3H-GnRH 的形成在培养基中。 GnRH 的分泌将通过灌注测定系统并测量胎盘组织响应的 GnRH 释放 60mM K+ 脉冲。 GnRH 在胎盘内的定位将是通过使用与 GnRH 抗体连接的免疫荧光来实现荧光化合物。最后，在第 12、18 和 28 天收集胎盘将分析妊娠期的 GnRH 含量。这些研究将扩展我们对维持妊娠机制的理解。此外，他们还将提供有关下丘脑外的新信息 GnRH 的来源。