SYNTHESIS AND SECRETION OF GNRH BY THE FETAL PLACENTA

胎儿胎盘合成和分泌 GNRH

• 批准号: 3317355
• 负责人: JANICE M BAHR
• 金额: $ 4.89万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3317355
• 关键词: cell type; chorionic gonadotropin; corpus luteum; gestational age; gonadotropin releasing factor; high performance liquid chromatography; hormone biosynthesis; immunofluorescence technique; immunoprecipitation; molecular sieving; organ culture; placenta; pregnancy; radioimmunoassay; statistics /biometry

In the pregnant rabbit doe, follicular estrogens and the fetal placenta are required to maintain progesterone production by the corpora lutea (CL) during gestation. The fetal placenta exerts its effects on the CL through either an antiluteolytic and/or luteotropic mechanism. However, studies examining the gonadotropin-like activity of rabbit placentas have failed to identify any chorionic gonadotropin. Recent evidence suggests that the placenta may act as an indirect luteotropin by modifying pituitary or follicular secretions. We have identified a small peptide with both biological and immunological GnRH-like activity in the rabbit placenta. It is not clear whether the placenta synthesizes this molecule or merely binds GnRH found in the peripheral circulation. The specific aims of this proposal are: 1) to determine whether the rabbit fetal placenta synthesizes GnRH; 2) to determine whether GnRH is secreted by the rabbit placenta; 3) to localize the site of synthesis or binding of GnRH within the placenta; and 4) to quantitate changes in the levels of GnRH in the placenta throughout pregnancy. Synthesis of GnRH will be determined by incubating placentas with 3H-leucine and measuring the formation of 3H-GnRH in culture medium. Secretion of GnRH will be determined using a perifusion system and measuring the release of GnRH by placental tissue in response to pulses of 60mM K+. Localization of GnRH within the placenta will be achieved through immunofluorescence using a GnRH antibody linked to a fluorescent compound. Finally, placentas collected at days 12, 18, and 28 of gestation will be analyzed for GnRH content. These studies will extend our understanding of mechanisms involved in the maintenance of pregnancy. In addition, they will provide new information concerning extrahypothalamic sources of GnRH.

在妊娠兔母鹿中，卵泡雌激素和胎儿胎盘 维持黄体（CL）产生孕酮所需 在怀孕期间 胎儿胎盘通过以下途径对CL发挥作用： 抗黄体溶解和/或促黄体机制。 然而研究 检测兔子胎盘的促性腺激素样活性， 找到绒毛膜促性腺激素 最近的证据表明， 胎盘可作为一种间接促黄体激素，通过调节垂体或 卵泡分泌物 我们发现了一种小肽， 兔胎盘中GnRH样生物学和免疫学活性。 它 尚不清楚胎盘是否合成这种分子或仅仅结合 外周循环中发现GnRH。 具体目标是 建议是：1）确定兔胎儿是否胎盘 合成GnRH; 2）确定GnRH是否由兔分泌 胎盘; 3）定位GnRH的合成或结合部位， 胎盘;和4）定量在GnRH水平的变化， 胎盘在整个怀孕期间。 GnRH的合成将通过以下方法测定： 用3 H-亮氨酸孵育胎盘并测量3 H-GnRH的形成 在培养基中。 将使用灌流法测定GnRH的分泌 系统和测量胎盘组织响应于 脉冲60 mM K+。 胎盘内GnRH的定位将 通过免疫荧光法，使用GnRH抗体连接到 荧光化合物 最后，在第12、18和28天收集胎盘 将分析妊娠期的GnRH含量。 这些研究将扩大 我们对维持妊娠机制的理解。 此外，他们将提供有关下丘脑外的新信息， GnRH的来源