EGG MATURATION: HORMONE RECEPTOR SITES AND UPTAKE

卵子成熟：激素受体位点和吸收

• 批准号: 3318294
• 负责人: THOMAS E SCHROEDER
• 金额: $ 9.37万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-30 至 1988-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3318294
• 关键词: adenine analog; autoradiography; cell cycle; chemical binding; cytoskeleton; egg /ovum; electron microscopy; fluorescence microscopy; hormone metabolism; membrane activity; microscopy; morphology; oogenesis; pinocytosis; receptor mediated endocytosis; saltwater environment; scintillation counter; videotape /videodisc

This project aims to investigate the early interactions between a defined hormone (l-methyladenine = 1-MA) and its target cell (starfish oocyte) that result in dramatic changes of the cytoskeleton and stimulate cell division. It is known that l-MA initially binds at the cell-surface of the oocyte and is transduced to they cytoplasma where is stimulates the completion of maturation divisions. This hormone-oocyte interaction, therefore, represents an important model system for studying hormone-cell surface interactions in general, as well as similar hormone-oocyte interactions in vertebrates including human, and the regulation of cell division. This project represents one of the Principal Investigator's long-term objectives: elucidation of the mechanisms of cell division. Experiments will test specific hypotheses concerning the mophological distribution of l-MA receptor sites on the oocyte surface and the cellular mechanism of l-MA uptake. They will employ molecular probes and markers in a set of morphological analyses. Tritiated-1-MA will be used to localize the initial binding sites of l-MA and reveal its intracellular pathway by autoradiography and light and electron microscopy. Fluid-content markers will be used to explore the existence, pathway and kinetics of endocytosis as the mechanism of hormone uptake in oocytes before and after application of l-MA; these agents include a fluorescent market (Lucifer Yellow CH) and a cytochemical marker (horseradish peroxidase) for microscopic analyses, and will be complemented by kinetic analyses of tritiated-insulin uptake using scintillation counting. These studies will reveal th role of receptor-mediated endocytosis in hormone-oocyte interations. Normal processes will be manipulated with two kinds of inhibitory drugs: antagonists of oocyte maturation and inhibitors of cytoskeletal organization and function. These manipulations will test the roles of the actin- and tubulin-based cytoskeletons in hormone transductiion and will explore the subcellular actions of known antagonists of oocyte maturation.

这个项目旨在研究一个定义的

项目成果

Contact-independent polarization of the cell surface and cortex of free sea urchin blastomeres.

游离海胆卵裂球细胞表面和皮层的接触无关极化。

• DOI: 10.1016/0012-1606(88)90209-6
• 发表时间: 1988
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Schroeder,TE

Fourth cleavage of sea urchin blastomeres: microtubule patterns and myosin localization in equal and unequal cell divisions.

海胆卵裂球的第四次分裂：等量和不等量细胞分裂中的微管模式和肌球蛋白定位。

• DOI: 10.1016/0012-1606(87)90454-4
• 发表时间: 1987
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Schroeder,TE