Designer DNA-binding factors

设计 DNA 结合因子

• 批准号: DP170101786
• 负责人: Prof Merlin Crossley
• 金额: $ 29.94万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP170101786
• 关键词: Designer; DNA; binding; factors

This project aims to use a natural transcription factor family to enhance the efficiency and functionality of designer DNA-binding factors. Research into the structure and function of zinc finger transcription factors, TAL effectors and CRISPR created designer DNA-binding factors. However, though research has improved the specificity of these factors’ genome-wide binding, their efficacy in regulating the expression of genes requires improvement. Using sequencing, the project intends to enhance the efficiency and function of these factors by designing modules to improve the stability of DNA binding and effectiveness in functionally regulating gene expression. The project outcomes could include knowledge enabling the use of genetically engineered DNA-binding proteins to artificially control gene expression, with significant scientific and economic implications.

该项目旨在利用一个天然转录因子家族来增强设计DNA结合因子的效率和功能。对锌指转录因子、TAL效应子和CRISPR的结构和功能的研究创造了设计师DNA结合因子。然而，尽管研究已经提高了这些因子在全基因组范围内结合的特异性，但它们在调节基因表达方面的功效还需要改进。利用测序，该项目旨在通过设计模块来提高这些因子的效率和功能，以提高DNA结合的稳定性和功能调节基因表达的有效性。该项目的成果可包括能够利用基因工程DNA结合蛋白人工控制基因表达的知识，具有重大的科学和经济影响。