Designer DNA-binding factors

本项目旨在利用天然转录因子家族来提高设计dna结合因子的效率和功能。研究锌指转录因子、TAL效应因子和CRISPR创造的设计dna结合因子的结构和功能。然而，虽然研究已经提高了这些因子全基因组结合的特异性，但其调节基因表达的功效还有待提高。本项目拟利用测序技术，通过设计模块来提高这些因子的效率和功能，以提高DNA结合的稳定性和功能调控基因表达的有效性。该项目的成果可能包括能够使用基因工程dna结合蛋白来人为控制基因表达的知识，具有重大的科学和经济意义。

This project aims to use a natural transcription factor family to enhance the efficiency and functionality of designer DNA-binding factors. Research into the structure and function of zinc finger transcription factors, TAL effectors and CRISPR created designer DNA-binding factors. However, though research has improved the specificity of these factors’ genome-wide binding, their efficacy in regulating the expression of genes requires improvement. Using sequencing, the project intends to enhance the efficiency and function of these factors by designing modules to improve the stability of DNA binding and effectiveness in functionally regulating gene expression. The project outcomes could include knowledge enabling the use of genetically engineered DNA-binding proteins to artificially control gene expression, with significant scientific and economic implications.