EOSINOPHIL MAJOR BASIC PROTEIN IN HUMAN PREGNANCY

人类妊娠期的嗜酸性粒细胞主要碱性蛋白

• 批准号: 3322871
• 负责人: Gerald J Gleich
• 金额: $ 13.97万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3322871
• 关键词: affinity chromatography; aminoacid analyzer; autoradiography; birth; blood chemistry; blood proteins; eclampsia; eosinophil; female; gel electrophoresis; gestational diabetes mellitus; high performance liquid chromatography; human pregnant subject; human subject; laboratory mouse; patient care management; placenta; pregnancy disorder; pregnancy toxemia /hypertension; premature labor; prenatal diagnosis; protein structure; radioimmunoassay; spontaneous abortion; trophoblast; urinalysis

Prior studies have shown that the eosinophil granule contains an intensely cationic proteinaceous molecule termed the major basic protein (gMBP). Guinea pig MBP accounts for over 50% of granule proteins and 25% of the total cellular protein. gMBP is localized to the core of the granule and exists there as a crystal. gMBP has also been identified in the human; both guinea pig and human gMBP are potent toxins to helminths and mammalian cells; and gMBP has been proposed as a mediator of pathological damage in bronchial asthma and other inflammatory diseases. During studies of the role of gMBP in hypersensitivity diseases, we found that elevated levels of gMBP are present in human pregnancy. MBP levels rise shortly after conception, plateau at about 20 weeks and show a late rise shortly before labor. The pregnancy-associated major basic protein (pMBP) behaves identically to gMBP in its immunochemical properties, but initial studies showed a difference in molecular weight. In recent studies, we have found that a late rise in pMBP levels is associated with human labor. In this proposal, we outline studies, first, to purify pMBP from placenta in quantities sufficient for chemical analyses. Second, we will test whether purified pMBP is identical to gMBP. These experiments are predicted on our ability to purify pMBP and on prior physicochemical information concerning gMBP. Third, we will measure levels of serum pMBP throughout human pregnancy in 100 normal pregnant women and in a control group of 30 nonpregnant women. And fourth, we will measure serum pMBP levels in various pathological conditions associated with pregnancy, including tests of the hypothesis that elevations of pMBP before 36 weeks predict premature labor. We also will test whether pMBP is useful in the management of prolonged pregnancy and whether a subset of patients with recurrent abortions may have abnormalities in the plasma levels of pMBP. Overall, these experiments will determine whether spontaneous labor can be predicted by late rises in pMBP and they offer the hope of obtaining new information concerning the function in human pregnancy of the relatively neglected trophoblast known as the X cell.

先前的研究表明，嗜酸性粒细胞颗粒含有一种 强烈的阳离子蛋白质分子被称为主要的碱性 蛋白（gMBP）。 豚鼠MBP占50%以上， 颗粒蛋白占细胞总蛋白的25%。 gMBP是 位于颗粒的核心并以晶体形式存在。 gMBP也已在人类中鉴定;豚鼠和 人gMBP是蠕虫和哺乳动物细胞的有效毒素; 和gMBP已被提出作为病理性的介导剂， 支气管哮喘和其他炎症性疾病的损害。 在研究gMBP在超敏反应性疾病中的作用期间， 我们发现在人类中gMBP的水平升高， 怀孕 MBP水平在受孕后不久上升， 大约20周，在临产前不久显示出晚升。 的 妊娠相关主要碱性蛋白（pMBP） 在其免疫化学性质上与gMBP相同，但初始 研究表明分子量不同。 近几 研究中，我们发现pMBP水平的后期上升是 与人类劳动有关。 在这个建议中，我们概述了研究，首先，从pMBP中纯化pMBP， 胎盘的数量足以进行化学分析。 第二、 我们将测试纯化的pMBP是否与gMBP相同。 这些 实验预测我们的能力，纯化pMBP和 关于gMBP的先前理化信息。 三是 将在整个人类妊娠期间测量血清pMBP水平 正常孕妇100例，对照组30例 未怀孕的女性 第四，我们将测量血清pMBP 在各种病理条件下的水平与 怀孕，包括测试的假设， 36周前pMBP可预测早产。 我们还将测试 pMBP是否适用于长期的管理 妊娠和是否有一个子集的患者复发 流产可能具有血浆pMBP水平的异常。 总的来说，这些实验将确定是否自发 分娩可以通过pMBP的后期上升来预测， 希望获得有关该功能的新信息， 人类怀孕的相对被忽视的滋养层称为 X细胞

项目成果

Biochemical and amino acid sequence analysis of human eosinophil granule major basic protein.

人嗜酸性粒细胞颗粒主要碱性蛋白的生化和氨基酸序列分析。

• 发表时间: 1988
• 期刊: The Journal of biological chemistry
• 作者: Wasmoen,TL;Bell,MP;Loegering,DA;Gleich,GJ;Prendergast,FG;McKean,DJ
• 通讯作者: McKean,DJ

Analysis of pregnancy-associated major basic protein levels throughout gestation.

分析整个妊娠期与妊娠相关的主要碱性蛋白水平。

• DOI: 10.1016/s0143-4004(05)80384-4
• 发表时间: 1993
• 期刊: Placenta
• 影响因子: 3.8
• 作者: Wagner,JM;Bartemes,K;Vernof,KK;Dunnette,S;Offord,KP;Checkel,JL;Gleich,GJ
• 通讯作者: Gleich,GJ

Evidence of eosinophil granule major basic protein in human placenta.

• DOI: 10.1084/jem.170.6.2051
• 发表时间: 1989-12-01
• 期刊: The Journal of experimental medicine
• 作者: Wasmoen TL;McKean DJ;Benirschke K;Coulam CB;Gleich GJ
• 通讯作者: Gleich GJ

Major basic protein as a marker of malignant potential in trophoblastic neoplasia.

主要碱性蛋白作为滋养细胞肿瘤恶性潜能的标志物。

• DOI: 10.1053/ob.1996.v175.a74413
• 发表时间: 1996
• 期刊: American journal of obstetrics and gynecology
• 影响因子: 9.8
• 作者: Gurian,KV;Podratz,KC;Elg,SA;Twiggs,LB;Lurain,JR;Wagner,JM;Gleich,GJ
• 通讯作者: Gleich,GJ

Localization of eosinophil-derived neurotoxin and eosinophil cationic protein in neutrophilic leukocytes.

嗜酸性粒细胞衍生的神经毒素和嗜酸性粒细胞阳离子蛋白在中性粒细胞中的定位。

• DOI: 10.1159/000024091
• 发表时间: 1999
• 期刊: International archives of allergy and immunology
• 影响因子: 2.8
• 作者: Sur,S;Glitz,DG;Kita,H;Kujawa,SM;Peterson,EA;Weiler,DA;Kephart,GM;Wagner,JM;George,TJ;Gleich,GJ;Leiferman,KM
• 通讯作者: Leiferman,KM