NEUTRAL PROTEINASES IN ENDOCHONDRAL BONE FORMATION

本项目旨在探讨神经蛋白酶在神经细胞凋亡中的作用。 去除生长区中伴随骨形成的软骨基质 胚胎肢原基的形成 对拟议的工作假设 研究表明，神经蛋白聚糖降解酶对组织抵抗， 和血清抑制剂，类似于恶性大鼠肿瘤分泌的一种 我们最近描述的一种酶可能参与了正常的 软骨去除伴随着胚胎中的软骨内骨形成。 对鸡胚肢体的初步研究表明， 生长区中的蛋白聚糖降解蛋白酶，其中之一可以 具有与肿瘤酶相似的性质。 1.用肿瘤酶作抗原的免疫定位方法， 单克隆抗体，将搜索类似的酶，在 胚胎大鼠长骨的生长区和骨修复组织中， 新生大鼠 2.发育中的鸡胚肢，可为 生物化学研究，将用于分离，纯化和 中性PG降解蛋白酶的表征。 3.这些酶的细胞来源、调节及其与骨的关系 将利用生物化学和免疫学的方法来探索其形成和修复 在这个实验室里建立了良好的方法。 所获得的知识可能有助于理解不仅正常 骨骼发育，还有遗传和代谢异常以及骨骼 修复过程。

This project is designed to explore the role of neural proteinases in the removal of cartilage matrix accompanying bone formation in the growth zones of embryonic limb primordia. The working hypothesis for the proposed studies is that neural proteoglycan degrading enzymes resistant to tissue and serum inhibitors, similar to one secreted by a malignant rat tumor enzyme recently described by us may be involved in the normal process of cartilage removal accompanying endochondral bone formation in the embryo. Preliminary studies in the chick embryo limb indicate the presence of proteoglycan degrading proteinases in the growth zone, one of which may have properties similar to that of the tumor enzyme. 1. By immunolocalization methods using the tumor enzyme as antigen for monoclonal antibodies a search will be made for similar enzymes in the growth zones of embryonic rat long bones and in bone repair tissue in neonatal rats. 2. Developing chick embryo limb, which can provide more tissue for biochemical studies, will be used for isolation, purification and characterization of neutral PG degrading proteinases. 3. Cellular source, regulation and relationship of these enzymes to bone formation and repair will be explored using biochemical and immunological methods well established in this Laboratory. The knowledge gained may be useful in understanding not only normal skeletal development, but also genetic and metabolic abnormalities and bone repair processes.