Deconvolving of Dynamic Cell Cycle Parameters from Flow Cytometry Data

从流式细胞术数据中解卷积动态细胞周期参数

• 批准号: EP/G037841/1
• 负责人: Rachel Errington
• 金额: $ 15.07万
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FG037841%2F1
• 关键词: Deconvolving; Dynamic; Cell; Cycle; Parameters

Cell cycle control systems change the way in which cells behave by: checking for the completion of vital tasks, choosing different routes through the cell cycle, determining how daughter cells inherit features, fixing the lifespan of a cell, or allowing a cell to react to its neighbours and the micro-environment. For a mathematical model of the cell cycle to hold credibility and usefulness within the science community, it has be of sufficient complexity to incorporate a minimum number of processes known to be involved in cell-cycle regulation, including growth and division, growth restriction, survival, programmed cell death, DNA checkpoint control and cellular damage response. Mathematical models capable of predicting such behaviour could contribute massively to research, reducing cost and time, reducing animal experimentation, and supporting clinical trials. We understand the wider scale of this endeavour and the need for biologists and theoreticians to work closely together to deliver both a specific and ambitious objective. This PDRA mobility award provides a discipline hopping opportunity to place a young talented engineer/physicist into a 'biology' laboratory where he can understand the extent and limitations of obtaining readouts from cells and thus how to apply advanced numerical solutions for interpreting these data, while at the same time ensuring the biologist has the means for independent validation and experimental testing.

细胞周期控制系统通过以下方式改变细胞的行为：检查重要任务的完成情况，在细胞周期中选择不同的路线，确定子细胞如何继承特征，固定细胞的寿命，或允许细胞对邻近细胞和微环境做出反应。为了让细胞周期的数学模型在科学界保持可信度和实用性，它必须具有足够的复杂性，以包含已知的参与细胞周期调控的最少数量的过程，包括生长和分裂、生长限制、生存、细胞程序性死亡、DNA检查点控制和细胞损伤反应。能够预测这种行为的数学模型可以为研究做出巨大贡献，减少成本和时间，减少动物实验，并支持临床试验。我们理解这一努力的更大规模，以及生物学家和理论家需要密切合作，以实现一个具体和雄心勃勃的目标。这个PDRA MOBILITY奖提供了一个学科跳跃的机会，让一位年轻的有才华的工程师/物理学家进入一个‘生物学’实验室，在那里他可以了解从细胞获取读数的程度和限制，从而了解如何应用高级数值解决方案来解释这些数据，同时确保生物学家拥有独立验证和实验测试的手段。