NUCLEO-CYTOPLASMIC INTERACTIONS IN EGGS AND OOCYTES

卵子和卵母细胞中的核质相互作用

• 批准号: 3316224
• 负责人: DAVID J NISHIOKA
• 金额: $ 5.2万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3316224
• 关键词: Asteroidea; autoradiography; cell fusion; cell growth regulation; cell nucleus; cytoplasm; egg /ovum; embryogenic cleavage; enzyme inhibitors; fertilization; nonmammalian vertebrate embryology; phase contrast microscopy; saltwater environment; sea urchins; systematic biology

The control of the cell cycle in early sea urchin and starfish embryos will be studied in terms of nucleo-cytoplasmic interactions. Aphidicolin, a recently discovered drug which inhibits DNA replication specifically, will be applied to early embryos in connection with the recently developed technique of egg fusion. This experimental approach is designed to make several new determinations about cell cycle control in the early embryo: (1) whether or not the unreplicated nucleus exerts negative control over progression through the cell cycle; (2) whether or not the cytoplasm of the replicated cell can exert positive control over the unreplicated nucleus; and (3) whether or not any controlling factors inferred from these studies can cross taxonomic class barriers between sea urchins and starfish. Additional new determinations about necleocytoplasmic interactions in unfertilized eggs and immature oocytes using the egg fusion technique only include: (4) whether or not nuclear DNA replication is stimulated in an unfertilized sea urchin egg upon fusion with a fertilized egg; (5) whether or not germinal vesicle breakdown and maturation of an immature sea urchin oocyte is stimulated upon fusion with an unfertilized egg; (6) whether or not germinal vesicle breakdown and maturation of an immature starfish oocyte is stimulated upon fusion with a mature starfish oocyte; and (7) whether or not any controlling factors inferred from these studies can cross taxonomic class barriers between sea urchins and starfish. These determinations should provide a more complete understanding of how the nucleus and cytoplasm are related with one another during the production of the egg and through early cleavage of the embryo.

海胆和海星早期胚胎细胞周期的控制 从核质相互作用的角度来研究。 阿非迪霉素a 最近发现的一种专门抑制DNA复制的药物， 应用于早期胚胎与最近开发的 卵子融合技术 这种实验方法旨在使 关于早期胚胎细胞周期控制的几个新决定： (1)未复制的细胞核是否对 （2）细胞周期的进展;（3）细胞质是否 复制细胞对未复制细胞核有正调控作用; 以及（3）是否有任何控制因素从这些研究中推断 可以跨越海胆和海星之间的分类障碍。 关于核质相互作用的其他新的确定， 未受精卵和未成熟卵母细胞仅使用卵融合技术 包括：（4）是否核DNA复制被刺激， 未受精的海胆卵与受精卵融合后;（5）是否 未成熟海胆的生殖囊泡破裂和成熟 卵母细胞在与未受精卵融合时受到刺激;（6）是否或 不是未成熟海星的萌发囊泡破裂和成熟 在与成熟海星卵母细胞融合后刺激卵母细胞;和（7） 从这些研究中推断出的任何控制因素是否可以 跨越海胆和海星之间的分类障碍。 这些 确定应该提供一个更完整的了解如何 细胞核和细胞质在生产过程中相互关联， 通过卵子和胚胎的早期卵裂。