HORMONAL REGULATION OF MONKEY SERTOLI CELLS IN CULTURE

培养中猴支持细胞的激素调节

• 批准号: 3317895
• 负责人: MADHWA H.G RAJ
• 金额: $ 12.93万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-08-01 至 1989-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3317895
• 关键词: Macaca; Mammalia; Sertoli cells; active immunization; affinity chromatography; androgen binding protein; androgens; animal age group; cow; cytology; electron microscopy; epididymis; fertility; follicle stimulating hormone; histochemistry /cytochemistry; hormone inhibitor; immunochemistry; inhibin; juvenile animal; laboratory rat; luteinizing hormone; mature animal; protein structure; radioimmunoassay; reproductive system pharmacology; semen; serum; sperm; spermatogenesis; spermicide; testis; testosterone; tissue /cell culture

The overall objective of this project is to evaluate the biochemical and morphological response of the testis and epididymis to a withdrawal or deprivation of FSH. The effects of treatment with inhibin on first wave of spermatogenesis in the immature rat as well as on spermatogenesis and fertility in the rat and monkey will be investigated. Levels of inhibin, Androgen Binding Protein (ABP), Testosterone, and DHT in the testis and epididymis will be measured in these animals. Circulating levels of gonadotropins, inhibin and androgens will be measured. Site of production of inhibin in rat and bovine Sertoli cells in culture and in bovine testis will be localized by electron microscopic immunocytochemical techniques. The effects of active immunization with FSH--subunit on the male crab-eating monkey (M. fasicularis) will be investigated, using the following parameters to monitor: a) changes in testicular morphology using light and electron microscopic techniques, b) semen analysis, including sperm count, motility, structure, levels of inhibin, and androgens, c) ABP levels in the testis and epididymis and d) peripheral levels of androgens, LH and any free residual FSH. The inhibin levels will be measured using radioimmunoassay and radioreceptor assay. The peripheral antibody titres in the plasma of the immunized monkeys will be correlated with the above parameters during the progress of immunization. Inhibin-FSH-Testosterone interrelations will be explored using antigonadotropic sera, and hypophysectomy in the rat and the monkey. These studies are of potential importance to a better understanding of the role of FSH in male reproduction. The proposed studies in inhibin will, in addition, provide new and significant information on the modulation of FSH secretion. These approaches hold the promise of providing effective means for pharmacologic blockade of FSH for both basic research and possible applications.

该项目的总体目标是评估生物化学和 睾丸和附睾对戒断的形态学反应，或 FSH缺乏。 利多卡因治疗对脑缺血第一波的影响 未成熟大鼠精子发生以及对精子发生和生育力的影响 在大鼠和猴中的作用。 雄激素结合水平 睾丸和附睾中的蛋白质（ABP）、睾酮和DHT将被 在这些动物中测量。 循环中促性腺激素、促性腺激素结合素和 将测量雄激素。 大鼠和牛体内抑制素的产生部位 培养物和牛睾丸中的支持细胞将通过电子显微镜定位 显微免疫细胞化学技术。 FSH-1亚单位主动免疫对雄蟹摄食的影响 猴（M.将使用以下参数进行研究， 监测：a）使用光和电子的睾丸形态学变化 显微镜技术，B）精液分析，包括精子计数，活力， c）睾丸中的ABP水平，以及 附睾和d）雄激素、LH和任何游离残留FSH的外周水平。 将使用放射免疫测定法和放射受体测定法测量Escherichia coli的水平。 比色法 免疫猴血浆中的外周抗体滴度 将与上述参数相关， 次免疫 将使用抗促性腺激素药物探索抑制素-FSH-睾酮的相互关系。 血清，以及大鼠和猴的垂体切除术。 这些研究对于更好地理解 FSH在男性生殖中的作用 此外，拟议中的在阿斯塔纳进行的研究将， 为FSH分泌调节提供了新的重要信息。 这些方法有望为药理学研究提供有效手段。 阻断FSH用于基础研究和可能的应用。