HORMONAL REGULATION OF SERTOLI CELLS IN CULTURE

培养中支持细胞的激素调节

• 批准号: 3317896
• 负责人: MADHWA H.G RAJ
• 金额: $ 2.47万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-08-01 至 1989-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3317896
• 关键词: Macaca; Mammalia; Sertoli cells; active immunization; affinity chromatography; androgen binding protein; androgens; animal age group; cow; cytology; electron microscopy; epididymis; fertility; follicle stimulating hormone; histochemistry /cytochemistry; hormone inhibitor; immunochemistry; inhibin; juvenile animal; laboratory rat; luteinizing hormone; mature animal; protein structure; radioimmunoassay; reproductive system pharmacology; semen; serum; sperm; spermatogenesis; spermicide; testis; testosterone; tissue /cell culture

The overall objective of this project is to evaluate the biochemical and morphological response of the testis and epididymis to a withdrawal or deprivation of FSH. The effects of treatment with inhibin on first wave of spermatogenesis in the immature rat as well as on spermatogenesis and fertility in the rat and monkey will be investigated. Levels of inhibin, Androgen Binding Protein (ABP), Testosterone, and DHT in the testis and epididymis will be measured in these animals. Circulating levels of gonadotropins, inhibin and androgens will be measured. Site of production of inhibin in rat and bovine Sertoli cells in culture and in bovine testis will be localized by electron microscopic immunocytochemical techniques. The effects of active immunization with FSH--subunit on the male crab-eating monkey (M. fasicularis) will be investigated, using the following parameters to monitor: a) changes in testicular morphology using light and electron microscopic techniques, b) semen analysis, including sperm count, motility, structure, levels of inhibin, and androgens, c) ABP levels in the testis and epididymis and d) peripheral levels of androgens, LH and any free residual FSH. The inhibin levels will be measured using radioimmunoassay and radioreceptor assay. The peripheral antibody titres in the plasma of the immunized monkeys will be correlated with the above parameters during the progress of immunization. Inhibin-FSH-Testosterone interrelations will be explored using antigonadotropic sera, and hypophysectomy in the rat and the monkey. These studies are of potential importance to a better understanding of the role of FSH in male reproduction. The proposed studies in inhibin will, in addition, provide new and significant information on the modulation of FSH secretion. These approaches hold the promise of providing effective means for pharmacologic blockade of FSH for both basic research and possible applications.

该项目的总体目标是评估生化和 睾丸和附睾对退出或退出的形态反应 剥夺 FSH。 抑制素治疗对第一波感染的影响 未成熟大鼠的精子发生以及精子发生和生育能力 将在大鼠和猴子中进行研究。 抑制素水平、雄激素结合 睾丸和附睾中的蛋白质 (ABP)、睾酮和 DHT 将 在这些动物身上进行了测量。 促性腺激素、抑制素和的循环水平 将测量雄激素。 大鼠和牛体内抑制素的产生场所 培养物和牛睾丸中的支持细胞将通过电子定位 显微免疫细胞化学技术。 FSH亚基主动免疫对雄性食蟹的影响 将使用以下参数对猴子（M. fasillaryis）进行研究 监测：a) 使用光和电子监测睾丸形态的变化 显微技术，b) 精液分析，包括精子计数、活力， 结构、抑制素水平和雄激素，c) 睾丸中的 ABP 水平和 附睾和 d) 雄激素、LH 和任何游离残留 FSH 的外周水平。 将使用放射免疫测定法和放射受体测量抑制素水平 化验。 免疫猴血浆中外周抗体滴度 在进行过程中会与上述参数相关 免疫接种。 将使用抗促性腺激素探索抑制素-FSH-睾酮之间的相互关系 大鼠和猴子的血清和垂体切除术。 这些研究对于更好地理解该角色具有潜在的重要性 FSH 在男性生殖中的作用。 此外，拟议的抑制素研究将 提供有关 FSH 分泌调节的新的重要信息。 这些方法有望为药理学提供有效的手段 封锁 FSH 用于基础研究和可能的应用。