A new tumor marker for Ovarian Cancer

卵巢癌的新肿瘤标志物

• 批准号: 6435489
• 负责人: MADHWA H.G RAJ
• 金额: $ 7.12万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6435489
• 关键词: age difference; binding proteins; biomarker; clinical research; diagnosis design /evaluation; female; gene expression; human subject; immunocytochemistry; menopause; menstrual cycle; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; neoplasm /cancer genetics; nutrition related tag; ovary neoplasms; racial /ethnic difference; riboflavin; tumor antigens; women's health

Cancer cells possess unique cellular signatures which signal the presence of the disease. This property can be potentially used as a marker to detect the presence of the cancer. Riboflavin Carrier Protein (RCP) is an estrogen induced growth and development specific protein. We hypothesize that serum levels of RCP can be used to accurately and non- invasively detect ovarian epithelial cancer and other cancers unique to women such as those of the breast, endometrium and cervix. In this project we will test this hypothesis using ovarian epithelial cancer as a model system. In a preliminary study of 6 women, we found that mean RCP levels were 3.5 ng/ml for state I and 11.2 ng.m. for state III ovarian cancer, as compared to 0.7 ng/ml for healthy subjects. Although the study size is small, the differences are highly statistically significant with p values for difference less than 0.05. In the present proposal, we will prospectively confirm our preliminary results in a case control study. We will recruit incident ovarian cancer cases from clinics at LSU Health Sciences Center, University Hospital and Memorial Medical Center. Control subjects not having any cancer will be recruited from Gynecology clinics and will be matched 1.1 for race, age, menopausal status and if pre-menopausal, for day of menstrual cycle. Serum RCP levels will be tested on both cases and controls at entry into the study. We will attempt to confirm our preliminary data that serum, RCP levels can accurately detect early ovarian epithelial cancer and predict its stage. We will also measure the serum levels of CA-125, a marker that is currently being used even though it is elevated in only about 50% of ovarian cancers. We will compare performance of RCP test with CA-125 test in order to evaluate the superiority of the former in predicting early ovarian cancer. We will compare performance of RCP test with CA-125 test in order to evaluate the superiority the former in predicting early ovarian cancer. Finally, using immunohistochemical techniques, we will characterize expression patterns of RCP in epithelial ovarian cancer tumor tissue. Our preliminary studies suggest a role of RCP as a molecular signature indicative of the present of ovarian cancer. The RCP test will be a valuable application of the use of cancer cell signatures as detection markers, which is a critical component of the 2001 Bypass Budget Document of NCI.

癌细胞具有独特的细胞特征，表明疾病的存在。该特性可潜在地用作检测癌症是否存在的标记。核黄素载体蛋白（RCP）是一种雌激素诱导生长和发育的特异性蛋白质。我们假设 RCP 的血清水平可用于准确、无创地检测卵巢上皮癌和女性特有的其他癌症，如乳腺癌、子宫内膜和宫颈癌。在这个项目中，我们将使用卵巢上皮癌作为模型系统来测试这一假设。在对 6 名女性进行的初步研究中，我们发现状态 I 的平均 RCP 水平为 3.5 ng/ml，状态 I 的平均 RCP 水平为 11.2 ng.m。对于 III 期卵巢癌，健康受试者的浓度为 0.7 ng/ml。尽管研究规模较小，但差异具有高度统计显着性，差异 p 值小于 0.05。在本提案中，我们将前瞻性地在病例对照研究中确认我们的初步结果。我们将从路易斯安那州立大学健康科学中心、大学医院和纪念医疗中心的诊所招募卵巢癌病例。将从妇科诊所招募没有患有任何癌症的对照受试者，并在种族、年龄、绝经状态以及绝经前的情况下对月经周期的日期进行 1.1 匹配。在进入研究时将对病例和对照进行血清 RCP 水平测试。我们将尝试证实我们的初步数据，即血清、RCP 水平可以准确检测早期卵巢上皮癌并预测其分期。我们还将测量 CA-125 的血清水平，这是一种目前正在使用的标记物，尽管它仅在约 50% 的卵巢癌中升高。我们将比较RCP测试与CA-125测试的性能，以评估前者在预测早期卵巢癌方面的优越性。我们将比较RCP测试与CA-125测试的性能，以评估前者在预测早期卵巢癌方面的优越性。最后，利用免疫组织化学技术，我们将表征上皮性卵巢癌肿瘤组织中 RCP 的表达模式。我们的初步研究表明 RCP 作为指示卵巢癌存在的分子特征的作用。 RCP 测试将是使用癌细胞特征作为检测标记的一个有价值的应用，这是 NCI 2001 年旁路预算文件的重要组成部分。