ULTRASONIC BIOEFFECTS ON MAMMALIAN DEVELOPMENT

拟议研究的长期目标是确定 超声波对哺乳动物的生物学影响 发展。建议的研究分为两组，第一组是 评价超声照射卵巢的效果，方法： 评估小鼠着床前胚胎发育。使用这个 模型中，已经设计了实验来检验不同 超声波水平对细胞分裂、DNA合成、蛋白质合成和 早期时间调控的细胞表面分子的出现 胚胎。 第二组实验是关于生物的 宫内植入对植入后胚胎的影响 暴露在超声波下。他们将检验超声波可以 对发育中的中枢神经系统造成不可逆转的损害。 已经设计了实验来表征由 超声波暴露，以确定损伤是否可逆，以证明 与超声分期相关的敏感性，并确定是否影响 是累积的。

The long-term objectives of the research proposed are to determine the biological consequences of exposure to ultrasound on mammalian development. The studies proposed fall into two groups, the first being to evaluate the effects of ultrasound exposure of the ovary, by means of assessing preimplantation embryonic development in the mouse. Using this model, experiments have been designed to examine the effects of different levels of ultrasound on cell division, DNA synthesis, protein synthesis and the appearance of temporally-regulated cell surface molecules in the early embryo. The second group of experiments is concerned with the biological consequences to the post-implantation embryo following intrauterine exposure to ultrasound. They will test the hypothesis that ultrasound can cause irreversible damage to the developing central nervous system. Experiments have been designed to characterize CNS damage resulting from ultrasound exposure, to determine if damage is reversible, to demonstrate stage-related susceptibility to ultrasound and to determine whether effects are cumulative.